Optoelectrokinetically-enabled diagnosis of diabetic retinopathy with dual biomarkers lipocalin 1 and tumor necrosis factor-α

Han Sheng Chuang, Hsiao Neng Lin, Jen Yi Wang

研究成果: Conference contribution

摘要

Diabetic retinopathy (DR) is an ocular morbidity that usually occurs in the retina of patients with chronic diabetes. Without proper medical intervention, DR eventually may lead to blindness. To meet the clinical need, this study developed an immunosensing technique based on optoelectrokinetically enabled beads for simultaneously detecting dual biomarkers, lipocalin 1 (LCN1) and tumor necrosis factor-α (TNF-α). A sandwiched configuration comprising capture antibody–antigen-probe antibody was formed by conjugating particles with capture antibodies. The additional dye-labeled secondary antibodies were eventually attached to the probe antibodies to determine the presence of the target biomarkers. The concentration of the target biomarkers was measured based on fluorescence intensity. The optoelectrokinetic technique, named as rapid electrokinetic patterning (REP), was used to enhance the fluorescence signal by concentrating the functionalized particles into a cluster. REP exhibited limit of detection as low as 110 pg/mL and was operated with simultaneous applications of optical illumination and electric field. Particles of different sizes were labeled with different dye colors to detect the corresponding DR biomarkers. The efficiency and accuracy of the proposed technique were increased by cross-matching multiple biomarkers in the tear of patients with DR. The optoelectrokinetic system can be used for treatments performed by ophthalmologists and can be extended to diagnose other oculopathies in the future.

原文English
主出版物標題22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
發行者Chemical and Biological Microsystems Society
頁面978-981
頁數4
ISBN(電子)9781510897571
出版狀態Published - 2018 1月 1
事件22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
持續時間: 2018 11月 112018 11月 15

出版系列

名字22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
2

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
國家/地區Taiwan
城市Kaohsiung
期間18-11-1118-11-15

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 生物工程
  • 化學工程(雜項)
  • 控制與系統工程

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