Optoelectrokinetically-Enabled Signal Enhancement for a Bead-Based FRET Fluorescence Immunoassay

Han Sheng Chuang, Kuan Chih Wang, Dar Bin Hsieh, Yong Qing Fu

研究成果: Conference contribution

摘要

Bead-based immunoassay has been growing as a promising technology in the point-of-care diagnostics due to great flexibility. For samples with dilute analytes, the colloidal particles can concentrate dispersed analytes and act as carriers for advanced particle manipulation. To realize a rapid in-situ immunoassay, Firster resonance energy transfer (FRET) was used herein to ensure only the target antigen lipocalin 1 (LCN1) to be detected. The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. In addition, an optoelectrokinetic technique was used to further concentrate the functionalized particles to enhance the FRET fluorescent signal. The detection was usually completed within several tens of seconds. A comparison between the based-based diagnostics coupled with the optoelectrokinetic technique and a conventional ELISA reader showed nearly 100-fold improvement of the bio-signal was made. The technique is expected to bring hopes for diagnosing diseases with dilute biomarkers in the early stage.

原文English
主出版物標題2014 International Symposium on Optomechatronic Technologies, ISOT 2014
發行者Institute of Electrical and Electronics Engineers Inc.
頁面101-104
頁數4
ISBN(電子)9781467367523
DOIs
出版狀態Published - 2014 1月 1
事件2014 International Symposium on Optomechatronic Technologies, ISOT 2014 - Seattle, United States
持續時間: 2014 11月 52014 11月 7

出版系列

名字2014 International Symposium on Optomechatronic Technologies, ISOT 2014

Other

Other2014 International Symposium on Optomechatronic Technologies, ISOT 2014
國家/地區United States
城市Seattle
期間14-11-0514-11-07

All Science Journal Classification (ASJC) codes

  • 機械工業
  • 電氣與電子工程

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