Orientation control of zebrafish embryos using artificial cilia in a 3D flow-through microchannel

Karthick Mani, Tsung Chun Chang Chien, Chia Yuan Chen

研究成果: Conference contribution

3 引文 斯高帕斯(Scopus)

摘要

The zebrafish has emerged as an important vertebrate model for genetic screens and new drug development due to its significant characteristics such as optical transparency, rapid ex vivo growth, and high genetic similarity to humans. Despite these benefits, the scale of zebrafish studies is still limited as a result of the lack of a robust method to manipulate zebrafish during screening. In this work a new microfluidic channel layout in conjunction with a series of magnetically actuated artificial cilia were employed to provide orientation control of zebrafish larvae with axial rotation capability. This method enables 0-15 degrees of rotation inside the microchannel with high accuracy and less detrimental impact, as opposed to the conventional methods. In addition, the bioactivity of tested larvas remains stable with no significant difference to those in the control group during the time-lapse imaging. The presented platform along with the provided analytical paradigm is forecasted to be beneficial to facilitate the zebrafish screening using microfluidics in pharmaceutical industry.

原文English
主出版物標題2015 IEEE 10th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2015
發行者Institute of Electrical and Electronics Engineers Inc.
頁面15-19
頁數5
ISBN(電子)9781467366953
DOIs
出版狀態Published - 2015 7月 1
事件10th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2015 - Xi'an, China
持續時間: 2015 4月 72015 4月 11

出版系列

名字2015 IEEE 10th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2015

Other

Other10th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2015
國家/地區China
城市Xi'an
期間15-04-0715-04-11

All Science Journal Classification (ASJC) codes

  • 控制與系統工程
  • 電氣與電子工程
  • 硬體和架構

指紋

深入研究「Orientation control of zebrafish embryos using artificial cilia in a 3D flow-through microchannel」主題。共同形成了獨特的指紋。

引用此