Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B

B. W. Lin, Y. C. Wang, P. Y. Chang-Liao, Y. J. Lin, S. T. Yang, J. H. Tsou, K. C. Chang, Y. W. Liu, J. T. Tseng, C. T. Lee, J. C. Lee, L. Y. Hung

研究成果: Article同行評審

9 引文 斯高帕斯(Scopus)

摘要

The chromosomal passenger complex (CPC) plays a pivotal role in controlling accurate chromosome segregation and cytokinesis during cell division. Aurora-B, one of the chromosomal passenger proteins, is important for the mitotic spindle assembly checkpoint (SAC). Previous reports noted that Aurora-C is predominantly expressed in male germ cells and has the same subcellular localization as Aurora-B. Increasing evidence indicates that Aurora-C is overexpressed in many somatic cancers, although its function is uncertain. Our previous study showed that the aberrant expression of Aurora-C increases the tumorigenicity of cancer cells. Here, we demonstrate that overexpressed Aurora-C displaces the centromeric localization of CPCs, including INCENP, survivin, and Aurora-B. When cells were treated with nocodazole to turn on SAC, both the Aurora-B protein stability and kinase activity were affected by overexpressed Aurora-C. As a result, the activation of spindle checkpoint protein, BubR1, and phosphorylation of histone H3 and MCAK were also eliminated in Aurora-C-overexpressing cells. Thus, our results suggest that aberrantly expressed Aurora-C in somatic cancer cells may impair SAC by displacing the centromeric localization of CPCs.

原文English
文章編號e1106
期刊Cell Death and Disease
5
發行號3
DOIs
出版狀態Published - 2014 三月

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

指紋 深入研究「Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B」主題。共同形成了獨特的指紋。

引用此