Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B

B. W. Lin, Y. C. Wang, P. Y. Chang-Liao, Y. J. Lin, S. T. Yang, J. H. Tsou, K. C. Chang, Y. W. Liu, J. T. Tseng, C. T. Lee, J. C. Lee, L. Y. Hung

研究成果: Article

9 引文 (Scopus)

摘要

The chromosomal passenger complex (CPC) plays a pivotal role in controlling accurate chromosome segregation and cytokinesis during cell division. Aurora-B, one of the chromosomal passenger proteins, is important for the mitotic spindle assembly checkpoint (SAC). Previous reports noted that Aurora-C is predominantly expressed in male germ cells and has the same subcellular localization as Aurora-B. Increasing evidence indicates that Aurora-C is overexpressed in many somatic cancers, although its function is uncertain. Our previous study showed that the aberrant expression of Aurora-C increases the tumorigenicity of cancer cells. Here, we demonstrate that overexpressed Aurora-C displaces the centromeric localization of CPCs, including INCENP, survivin, and Aurora-B. When cells were treated with nocodazole to turn on SAC, both the Aurora-B protein stability and kinase activity were affected by overexpressed Aurora-C. As a result, the activation of spindle checkpoint protein, BubR1, and phosphorylation of histone H3 and MCAK were also eliminated in Aurora-C-overexpressing cells. Thus, our results suggest that aberrantly expressed Aurora-C in somatic cancer cells may impair SAC by displacing the centromeric localization of CPCs.

原文English
文章編號e1106
期刊Cell Death and Disease
5
發行號3
DOIs
出版狀態Published - 2014 三月

指紋

M Phase Cell Cycle Checkpoints
Aurora Kinase B
Nocodazole
Neoplasms
Chromosome Segregation
Cytokinesis
Protein Stability
Germ Cells
Cell Division
Histones
Protein Kinases
Proteins
Phosphorylation

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

引用此文

@article{82d99ff9b88646a7a7db05dec5bb6257,
title = "Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B",
abstract = "The chromosomal passenger complex (CPC) plays a pivotal role in controlling accurate chromosome segregation and cytokinesis during cell division. Aurora-B, one of the chromosomal passenger proteins, is important for the mitotic spindle assembly checkpoint (SAC). Previous reports noted that Aurora-C is predominantly expressed in male germ cells and has the same subcellular localization as Aurora-B. Increasing evidence indicates that Aurora-C is overexpressed in many somatic cancers, although its function is uncertain. Our previous study showed that the aberrant expression of Aurora-C increases the tumorigenicity of cancer cells. Here, we demonstrate that overexpressed Aurora-C displaces the centromeric localization of CPCs, including INCENP, survivin, and Aurora-B. When cells were treated with nocodazole to turn on SAC, both the Aurora-B protein stability and kinase activity were affected by overexpressed Aurora-C. As a result, the activation of spindle checkpoint protein, BubR1, and phosphorylation of histone H3 and MCAK were also eliminated in Aurora-C-overexpressing cells. Thus, our results suggest that aberrantly expressed Aurora-C in somatic cancer cells may impair SAC by displacing the centromeric localization of CPCs.",
author = "Lin, {B. W.} and Wang, {Y. C.} and Chang-Liao, {P. Y.} and Lin, {Y. J.} and Yang, {S. T.} and Tsou, {J. H.} and Chang, {K. C.} and Liu, {Y. W.} and Tseng, {J. T.} and Lee, {C. T.} and Lee, {J. C.} and Hung, {L. Y.}",
year = "2014",
month = "3",
doi = "10.1038/cddis.2014.37",
language = "English",
volume = "5",
journal = "Cell Death and Disease",
issn = "2041-4889",
publisher = "Nature Publishing Group",
number = "3",

}

Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B. / Lin, B. W.; Wang, Y. C.; Chang-Liao, P. Y.; Lin, Y. J.; Yang, S. T.; Tsou, J. H.; Chang, K. C.; Liu, Y. W.; Tseng, J. T.; Lee, C. T.; Lee, J. C.; Hung, L. Y.

於: Cell Death and Disease, 卷 5, 編號 3, e1106, 03.2014.

研究成果: Article

TY - JOUR

T1 - Overexpression of Aurora-C interferes with the spindle checkpoint by promoting the degradation of Aurora-B

AU - Lin, B. W.

AU - Wang, Y. C.

AU - Chang-Liao, P. Y.

AU - Lin, Y. J.

AU - Yang, S. T.

AU - Tsou, J. H.

AU - Chang, K. C.

AU - Liu, Y. W.

AU - Tseng, J. T.

AU - Lee, C. T.

AU - Lee, J. C.

AU - Hung, L. Y.

PY - 2014/3

Y1 - 2014/3

N2 - The chromosomal passenger complex (CPC) plays a pivotal role in controlling accurate chromosome segregation and cytokinesis during cell division. Aurora-B, one of the chromosomal passenger proteins, is important for the mitotic spindle assembly checkpoint (SAC). Previous reports noted that Aurora-C is predominantly expressed in male germ cells and has the same subcellular localization as Aurora-B. Increasing evidence indicates that Aurora-C is overexpressed in many somatic cancers, although its function is uncertain. Our previous study showed that the aberrant expression of Aurora-C increases the tumorigenicity of cancer cells. Here, we demonstrate that overexpressed Aurora-C displaces the centromeric localization of CPCs, including INCENP, survivin, and Aurora-B. When cells were treated with nocodazole to turn on SAC, both the Aurora-B protein stability and kinase activity were affected by overexpressed Aurora-C. As a result, the activation of spindle checkpoint protein, BubR1, and phosphorylation of histone H3 and MCAK were also eliminated in Aurora-C-overexpressing cells. Thus, our results suggest that aberrantly expressed Aurora-C in somatic cancer cells may impair SAC by displacing the centromeric localization of CPCs.

AB - The chromosomal passenger complex (CPC) plays a pivotal role in controlling accurate chromosome segregation and cytokinesis during cell division. Aurora-B, one of the chromosomal passenger proteins, is important for the mitotic spindle assembly checkpoint (SAC). Previous reports noted that Aurora-C is predominantly expressed in male germ cells and has the same subcellular localization as Aurora-B. Increasing evidence indicates that Aurora-C is overexpressed in many somatic cancers, although its function is uncertain. Our previous study showed that the aberrant expression of Aurora-C increases the tumorigenicity of cancer cells. Here, we demonstrate that overexpressed Aurora-C displaces the centromeric localization of CPCs, including INCENP, survivin, and Aurora-B. When cells were treated with nocodazole to turn on SAC, both the Aurora-B protein stability and kinase activity were affected by overexpressed Aurora-C. As a result, the activation of spindle checkpoint protein, BubR1, and phosphorylation of histone H3 and MCAK were also eliminated in Aurora-C-overexpressing cells. Thus, our results suggest that aberrantly expressed Aurora-C in somatic cancer cells may impair SAC by displacing the centromeric localization of CPCs.

UR - http://www.scopus.com/inward/record.url?scp=84897370555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897370555&partnerID=8YFLogxK

U2 - 10.1038/cddis.2014.37

DO - 10.1038/cddis.2014.37

M3 - Article

C2 - 24603334

AN - SCOPUS:84897370555

VL - 5

JO - Cell Death and Disease

JF - Cell Death and Disease

SN - 2041-4889

IS - 3

M1 - e1106

ER -