p53 amino acids 339-346 represent minimal p53 repression domain

Tse Ming Hong, Jeremy J.W. Cheni, Konan Peck, Pan Chyr Yang, Cheng Wen Wu

研究成果: Article

17 引文 (Scopus)

摘要

The p53 tumor suppressor protein functions as an activator and also as a repressor of gene transcription. Currently, the mechanism of transcriptional repression by p53 remains poorly understood. To help clarify this mechanism, we carried out studies designed to identify the minimal repression domain that inhibits p53 transcriptional activities. We found only eight amino acids (339-346) of the COOH-terminal domain (termed P53MRD) that possess activities of repression. The exact location of this minimal domain is on the E6-binding region, and it lacks the ability of tetramerization. P53MRD is able to repress the transcription of p53 while not affecting VP16. The mutants (amino acids M349P and F341D) of native p53 also lost transcriptional repression of the thymidine kinase chloramphenicol acetyltransferase promoter. These results suggest that this eight-amino acid element is required for the repression of p53.

原文English
頁(從 - 到)1510-1515
頁數6
期刊Journal of Biological Chemistry
276
發行號2
DOIs
出版狀態Published - 2001 一月 12

指紋

Transcription
Amino Acids
Activator Appliances
Tumor Suppressor Protein p53
Chloramphenicol O-Acetyltransferase
Thymidine Kinase
Genes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

引用此文

Hong, Tse Ming ; Cheni, Jeremy J.W. ; Peck, Konan ; Yang, Pan Chyr ; Wu, Cheng Wen. / p53 amino acids 339-346 represent minimal p53 repression domain. 於: Journal of Biological Chemistry. 2001 ; 卷 276, 編號 2. 頁 1510-1515.
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p53 amino acids 339-346 represent minimal p53 repression domain. / Hong, Tse Ming; Cheni, Jeremy J.W.; Peck, Konan; Yang, Pan Chyr; Wu, Cheng Wen.

於: Journal of Biological Chemistry, 卷 276, 編號 2, 12.01.2001, p. 1510-1515.

研究成果: Article

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AU - Wu, Cheng Wen

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