p53 overexpression and downregulation of inter-α-inhibitor are associated with hyaluronidase enhancement of TNF cytotoxicity in L929 fibroblasts

Nan Shan Chang, Gregory Carey, Nicole Pratt, Elaina Chu, Melody Ou

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Degradation of extracellular matrix by hyaluronidase increases murine L929 cell sensitivity to tumor necrosis factor (TNF) cytotoxicity. Seeding and culturing L929 cells onto the matrix of serum fetuin and the hyaluronate-binding inter-α-inhibitor resulted in inhibition of hyaluronidase-enhanced TNF killing, suggesting that the release of these proteins from hyaluronidase-degraded matrix confers cellular TNF susceptibility. Metabolic labeling studies showed that hyaluronidase mediated de novo protein synthesis and downregulated several proteins in L929 cells. Specifically, hyaluronidase upregulated p53 protein expression (>200%) but downregulated a p85 inter-α-inhibitor-like protein (>90%) in L929 cells, whereas it had no effect on the protein levels of ICH-1, Bcl-xL, Bcl-2, Fas ligand, CAS (cellular apoptosis susceptible protein), TIAR (an RNA-binding protein) and α-tubulin. Conceivably, hyaluronidase enhancement of TNF sensitivity in L929 cells is p53-dependent and the matrix inter-α-inhibitor contributes a protective role against TNF cytotoxicity. Copyright (C) 1998 Elsevier Science Ireland Ltd.

原文English
頁(從 - 到)45-54
頁數10
期刊Cancer Letters
131
發行號1
DOIs
出版狀態Published - 1998 九月 11

All Science Journal Classification (ASJC) codes

  • 腫瘤科
  • 癌症研究

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