Paclitaxel-loaded stabilizer-free poly(D,L-lactide-co-glycolide) nanoparticles conjugated with quantum dots for reversion of anti-cancer drug resistance and cancer cellular imaging

Wen Shuo Kuo, Yu Cian Ku, Hei Tin Sei, Fong Yu Cheng, Chen-Sheng Yeh

研究成果: Article

15 引文 (Scopus)

摘要

We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.

原文English
頁(從 - 到)923-934
頁數12
期刊Journal of the Chinese Chemical Society
56
發行號5
DOIs
出版狀態Published - 2009 一月 1

指紋

Paclitaxel
Semiconductor quantum dots
Nanoparticles
Imaging techniques
Pharmaceutical Preparations
Cells
Cytotoxicity
polylactic acid-polyglycolic acid copolymer
Drug Carriers
P-Glycoprotein
Cell membranes
Verapamil
Curing

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

引用此文

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abstract = "We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.",
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AU - Kuo, Wen Shuo

AU - Ku, Yu Cian

AU - Sei, Hei Tin

AU - Cheng, Fong Yu

AU - Yeh, Chen-Sheng

PY - 2009/1/1

Y1 - 2009/1/1

N2 - We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.

AB - We prepared the PLGA-loaded anti-cancer drug and coated it with quantum dots to make it a dual-function nanoparticles, and analyzed its potential use in cellular imaging and curing cancers. Two cancer cell lines, paclitaxel-sensitive KB and paclitaxel-resistant KB paclitaxel-50 cervical carcinoma cells, were the relativistic models for analysis of the cytotoxicity of free paclitaxel and paclitaxel-loaded PLGA conjugated with quantum-dot nanoparticles. The paclitaxel-loaded PLGA conjugated with quantum dots nanoparticles were significantly more cytotoxic than the free paclitaxel drug in paclitaxel-resistant KB paclitaxel-50 cells. This might have been because the cancer cells developed multi-drug resistance (MDR), which hampered the action of free paclitaxel by pumping its molecules to extracellular areas. Addition of verapamil, a P-glycoprotein inhibitor, reversed the MDR mechanism and significantly reduced KB paclitaxel-50 cell viability. As a result, KB paclitaxel-50 was highly associated with MDR on the cell membrane. The cytotoxicity results indicated that PLGA nanoparticles served as drug carriers and protected the drugs from MDR-accelerated efflux. Combined quantum dots with PLGA nanoparticles allowed additional functionality for cellular imaging.

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