A method is proposed for detecting microparticles in a microflow cytometer by means of small angle forward-scattered light measurements. The proposed cytometer comprises a commercial photovoltaic cell, an adjustable power laser module, and a PDMS microfluidic chip. The detection performance of the proposed device is evaluated using particles with dimensions of 5, 8, 10, and 15 μm, respectively, given forward-light scattering angles of 5 and 8° and laser powers ranging from 15-25 mW. It is shown that for a constant laser power and particle size, the S/N of the detected light signal increases with a reducing forward-scattering angle. Moreover, for a constant forward-scattering angle and particle size, the S/N increases with an increasing laser power. The intensity of the forward-scattered light signal is found to vary linearly with the particle size and has a correlation coefficient of R2 = 0.967, 0.967, and 0.963 given laser powers of 15, 20, and 25 mW, respectively, and a forward-scattering angle of 5°. Moreover, the CV of the forward-scattered light intensity is found to lie within the range of 20-30% for both forward-scattering angles. Overall, the present results suggest that the proposed device has significant potential for detection applications in the medical, environmental monitoring, and biological science fields.
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Clinical Biochemistry