Patch-clamp study on ion channels in the tonoplast of nitellopsis obtusa

Maki Katsuhara, Tetsuro Mimura, Masashi Tazawa

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8 引文 斯高帕斯(Scopus)

摘要

Cytoplasmic drops covered with the tonoplast were prepared from internodal cells of Nitellopsis grown in fresh water. Applying the patch-clamp technique and the microinjection technique to such drops, we characterized the ion channels in the tonoplast. Both in cell-free patches and in the cytoplasmic-drop-attached patches, the tonoplast K+ channel was identified. The permeability ratio between Na+ and K+ was calculated to be 0.2. This channel would provide a molecular basis for the Na+/K+ exchange at the tonoplast. In cell-free patches, the K+channel was not activated by Ca2+. However, in the case of attached patches, microinjection of Ca2+ into a drop activated the K+ channel with a lag of a few seconds, suggesting that some cytoplasmic factor(s) may mediate the activation of the K+ channel by Ca2+. The conductance of this channel was not changed by cytoplasmic Ca2+, but the probability of opening increased markedly. In addition to the K+ channel, a second type of channel was also identified in cell-free patches. This channel may be the Cl- channel.

原文English
頁(從 - 到)179-184
頁數6
期刊Plant and Cell Physiology
32
發行號2
DOIs
出版狀態Published - 1991 3月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 植物科學
  • 細胞生物學

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