Pathogen detection from phalaenopsis orchids by using an integrated microfluidic system

Wen Hsin Chang, Sung Yi Yang, Chih Hung Wang, Gwo Bin Lee, Tzong-Yueh Chen, Ping Chen Li, Fuh Jyh Jan

研究成果: Conference contribution

2 引文 斯高帕斯(Scopus)

摘要

Early detection of pathogens in high-value agricultural species is crucial. Therefore., many methods which can detect agricultural pathogens to prevent economic loss have been developed. Among them., immunoassays., nucleic acid hybridization and polymerase chain reaction have been demonstrated to detect pathogens in Phalaenopsis orchid successfully with satisfactory sensitivity and specificity. However., above-mentioned methods all have some disadvantages including lengthy process or requiring specialized laboratory facilities and well-trained technicians. The current study therefore presents an integrated micro fluidic system for rapid and automatic detection of pathogens in agricultural species. The entire procedure., including pathogen-specific ribonucleic acid (RNA) purification., nucleic acid amplification using reverse transcription loop-mediated-isothermal-amplification (RT-LAMP) and optical detection., can be automatically performed on a single chip within 65 minutes. Moreover., the detection module can be interchanged between a fluorescent detector and a turbidity detector., depending on the need of the operator. Furthermore., the developed system can detect pathogens directly from fresh agriculture tissues such as leaves and flowers of the Phalaneopsis orchids. This is the first time that an interchangeable integrated micro fluidic system for the detection of Phalaneopsis orchids has been demonstrated. Some of the most prevalent Phalaenopsis orchid pathogens., such as Cymbidium mosaic virus (CymMV) and Tomato spotted wilt virus (TSWV) for Phalaneopsis orchids were used in the current study to demonstrate the capabilities of the developed system. It is concluded that this system with dual detection units can directly detect pathogens from crude agricultural materials successfully.

原文English
主出版物標題NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
頁面6-10
頁數5
DOIs
出版狀態Published - 2012 12月 1
事件6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
持續時間: 2012 11月 42012 11月 7

出版系列

名字IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN(列印)2159-6964
ISSN(電子)2159-6972

Other

Other6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
國家/地區Thailand
城市Bangkok
期間12-11-0412-11-07

All Science Journal Classification (ASJC) codes

  • 分子醫學
  • 生物醫學工程
  • 微生物學(醫學)

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