Pea chloroplast DnaJ-J8 and Toc12 are encoded by the same gene and localized in the stroma

Chi Chou Chiu, Lih Jen Chen, Hsou Min Li

研究成果: Article同行評審

21 引文 斯高帕斯(Scopus)

摘要

Toc12 is a novel J domain-containing protein identified in pea (Pisum sativum) chloroplasts. It was shown to be an integral outer membrane protein localizing in the intermembrane space of the chloroplast envelope. Furthermore, Toc12 was shown to associate with an intermembrane space Hsp70, suggesting that Toc12 is important for protein translocation across the chloroplast envelope. Toc12 shares a high degree of sequence similarity with Arabidopsis (Arabidopsis thaliana) DnaJ-J8, which has been suggested to be a soluble protein of the chloroplast stroma. Here, we isolated genes encoding DnaJ-J8 from pea and found that Toc12 is a truncated clone of one of the pea DnaJ-J8s. Protein import analyses indicate that Toc12 and DnaJ-J8s possess a cleavable transit peptide and are localized in the stroma. Arabidopsis mutants with T-DNA insertions in the DnaJ-J8 gene show no defect in chloroplast protein import. Implications of these results in the energetics and mechanisms of chloroplast protein import are discussed.

原文English
頁(從 - 到)1172-1182
頁數11
期刊Plant Physiology
154
發行號3
DOIs
出版狀態Published - 2010 十一月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 遺傳學
  • 植物科學

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