Pedv infection generates conformation-specific antibodies that can be effectively detected by a cell-based elisa

Wei Ting Hsu, Chia Yu Chang, Chih Hsuan Tsai, Sung Chan Wei, Huei Ru Lo, Robert John S. Lamis, Hui Wen Chang, Yu Chan Chao

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes serious and highly con-tagious enteric disease in swine worldwide. In this study, we constructed a recombinant baculovirus (S-Bac) expressing full-length spike protein of the virulent epidemic genotype 2b (G2b) PEDV strain for serological studies of infected pigs. We found that most spike-specific antibodies produced upon PEDV infection in pigs are conformation-specific and they could be detected on S-Bac-infected insect cells by immunofluorescent assay, but they were insensitive to Western blot analysis, the typical method for antiserum analysis. These results indicated that spike conformation is crucial for serum recognition. Since it is difficult to purify trimeric spike membrane protein for conventional enzyme-linked immunosorbent assay (ELISA), we used S-Bac to generate a novel cell-based ELISA for convenient PEDV detection. We analyzed 100 pig serum samples, and our cell-based ELISA exhibited a sensitivity of 100%, a specificity of 97%, and almost perfect agreement [Cohen’s kappa coefficient value (κ) = 0.98] with immunocytochemical staining results. Our cell-based ELISA rapidly presented antigen for proper detection of conformation-specific antibodies, making PEDV detection more convenient, and it will be useful for detecting many viral diseases in the future.

原文English
文章編號303
期刊Viruses
13
發行號2
DOIs
出版狀態Published - 2021 2月

All Science Journal Classification (ASJC) codes

  • 傳染性疾病
  • 病毒學

指紋

深入研究「Pedv infection generates conformation-specific antibodies that can be effectively detected by a cell-based elisa」主題。共同形成了獨特的指紋。

引用此