Phosphate transport and apoplastic phosphate homeostasis in barley leaves

Tetsuro Mimura, Zu Hua Yin, Eva Wirth, Karl Josef Dietz

研究成果: Article同行評審

21 引文 斯高帕斯(Scopus)


Levels of apoplastic inorganic phosphate (Pi) in leaves and Pi-transport activities of mesophyll cells were measured in situ in control and Pi-deficient plants. When detached leaves were fed a solution that contained 10 mM Pi, the apoplastic Pi levels, as measured by an infiltration method, remained almost constant. When the leaves were immersed in pure water, the apoplastic Pi level gradually decreased. With 50 mM Pi in the feeding solution, the level increased dramatically. The apoplastic Pi levels in Pi-deficient leaves were somewhat, but not very much lower than those in controls. When the immersion medium was changed to pure water 60 mm after feeding with 10 mM Pi, the apoplastic Pi levels started to decrease and then returned to the initial level. It is suggested that intracellular Pi may be transported back to the apoplast to maintain the apoplastic Pi levels at a constant value.Changes in cytoplasmic pH were measured during feeding of Pi to the leaves by use of the pH-sensitive fluorescent dye, pyranine after Yin et al. (l990a, b). On feeding of Pi the cytoplasmic pH decreased in Pi-deficient plants as a result of co-transport of Pi and protons in situ. After removal of Pi from the immersion medium, the cytoplasmic pH returned to the original value.

頁(從 - 到)563-568
期刊Plant and Cell Physiology
出版狀態Published - 1992 7月

All Science Journal Classification (ASJC) codes

  • 生理學
  • 植物科學
  • 細胞生物學


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