Phosphorylation of LCRMP-1 by GSK3β promotes filopoda formation, migration and invasion abilities in lung cancer cells

Wen Lung Wang, Tse Ming Hong, Yih Leong Chang, Chen Tu Wu, Szu Hua Pan, Pan Chyr Yang

研究成果: Article

10 引文 (Scopus)

摘要

LCRMP-1, a novel isoform of CRMP-1, can promote cancer cell migration, invasion and associate with poor clinical outcome in patients with non-small-cell lung cancer (NSCLC). However, the underlying regulatory mechanisms of LCRMP-1 in cancer cell invasiveness still remain obscure. Here, we report that GSK3β can phosphorylate LCRMP-1 at Thr-628 in consensus sequences and this phosphorylation is crucial for function of LCRMP-1 to promote filopodia formation, migration and invasion in cancer cells. Impediment of Thr-628 phosphorylation attenuates the stimulatory effects of LCRMP-1 on filopodia forming, migration and invasion abilities in cancer cells; simultaneously, kinase-dead GSK3β diminishes regulation of LCRMP-1 on cancer cell invasion. Furthermore, we also found that patients with low-level Ser-9-phosphorylated GSK3β expression and high-level LCRMP-1 expression have worse overall survival than those with high-level inactive GSK3β expressions and low-level LCRMP-1 expressions (P<0.0001). Collectively, these results demonstrate that GSK3β-dependent phosphorylation of LCRMP-1 provides an important mechanism for regulation of LCRMP-1 on cancer cell invasiveness and clinical outcome.

原文English
文章編號e31689
期刊PloS one
7
發行號2
DOIs
出版狀態Published - 2012 二月 21

指紋

Phosphorylation
lung neoplasms
Lung Neoplasms
phosphorylation
Cells
pseudopodia
Neoplasms
Pseudopodia
cell invasion
consensus sequence
Consensus Sequence
cell movement
Non-Small Cell Lung Carcinoma
Cell Movement
neoplasm cells
Protein Isoforms
phosphotransferases (kinases)
Phosphotransferases
Survival

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

引用此文

Wang, Wen Lung ; Hong, Tse Ming ; Chang, Yih Leong ; Wu, Chen Tu ; Pan, Szu Hua ; Yang, Pan Chyr. / Phosphorylation of LCRMP-1 by GSK3β promotes filopoda formation, migration and invasion abilities in lung cancer cells. 於: PloS one. 2012 ; 卷 7, 編號 2.
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abstract = "LCRMP-1, a novel isoform of CRMP-1, can promote cancer cell migration, invasion and associate with poor clinical outcome in patients with non-small-cell lung cancer (NSCLC). However, the underlying regulatory mechanisms of LCRMP-1 in cancer cell invasiveness still remain obscure. Here, we report that GSK3β can phosphorylate LCRMP-1 at Thr-628 in consensus sequences and this phosphorylation is crucial for function of LCRMP-1 to promote filopodia formation, migration and invasion in cancer cells. Impediment of Thr-628 phosphorylation attenuates the stimulatory effects of LCRMP-1 on filopodia forming, migration and invasion abilities in cancer cells; simultaneously, kinase-dead GSK3β diminishes regulation of LCRMP-1 on cancer cell invasion. Furthermore, we also found that patients with low-level Ser-9-phosphorylated GSK3β expression and high-level LCRMP-1 expression have worse overall survival than those with high-level inactive GSK3β expressions and low-level LCRMP-1 expressions (P<0.0001). Collectively, these results demonstrate that GSK3β-dependent phosphorylation of LCRMP-1 provides an important mechanism for regulation of LCRMP-1 on cancer cell invasiveness and clinical outcome.",
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Phosphorylation of LCRMP-1 by GSK3β promotes filopoda formation, migration and invasion abilities in lung cancer cells. / Wang, Wen Lung; Hong, Tse Ming; Chang, Yih Leong; Wu, Chen Tu; Pan, Szu Hua; Yang, Pan Chyr.

於: PloS one, 卷 7, 編號 2, e31689, 21.02.2012.

研究成果: Article

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