Phosphorylation of proteins in Xanthomonas campestris pv. oryzae

Bei chang Yang, Yi fang Ho, Jun shang Liu, Tsong teh Kuo

研究成果: Article同行評審

3 引文 斯高帕斯(Scopus)


Protein phosphorylation was studied in Xanthomonas campestris pv. oryzae in vivo and in vitro. In vitro labelling showed that the protein kinases in this bacterium used both ATP and GTP as nucleotide substrates at nearly the same efficiency. At least 6 proteins were phosphorylated in vitro, including abundant species of p81, p44, and p32 with Mr of 81000, 44000, and 32000, respectively. Three types of phosphate-protein linkage were found in this bacterium: O-phosphate, N-phosphate and probably acyl phosphate. The p81 and p32 were phosphorylated at histidine. The p44 had mainly phosphoserine and a small part of phosphohistidine. The phosphorylation profile was variable depending on the growth conditions. Furthermore, by a virulent phage Xp10 infection the quantity of phosphorylation increased: for phosphohistinine more than 10-fold, and for phosphoserine about 3-fold. Thus, in this bacterium phosphorylation may be linked with a physiological regulation system and with Xp10 phage development.

頁(從 - 到)262-266
期刊Archives of Microbiology
出版狀態Published - 1992 9月 1

All Science Journal Classification (ASJC) codes

  • 微生物學
  • 生物化學
  • 分子生物學
  • 遺傳學


深入研究「Phosphorylation of proteins in Xanthomonas campestris pv. oryzae」主題。共同形成了獨特的指紋。