Photodynamic inactivation of methicillin-resistant Staphylococcus aureus by indocyanine green and near infrared light

Tak-Wah Wong, En Chi Wu, Wen-Chien Ko, Ching Chi Lee, Lien I. Hor, I-Hsiu Huang

研究成果: Article

3 引文 (Scopus)

摘要

Background: Antimicrobial photodynamic therapy (APDT) has become a potential regimen to treat multidrug-resistant bacterial infections. Limited data showed indocyanine green (ICG), a safe and inexpensive contrast medium for eye angiography and hepatic function examination, is an effective photosensitizer in APDT to kill methicillin-resistant Staphylococcus aureus (MRSA) after excitation with laser. Objective: We investigated the potentials of ICG-APDT with an inexpensive, non-coherent commercial near infrared (NIR) lamp against MRSA. Methods: The inhibition of MRSA was studied after exposing bacteria to NIR with different light doses and concentrations of ICG. The selectivity on MRSA was examined on human fibroblasts. Bacterial virulence including the activities of coagulase and enterotoxin was investigated. The effects of singlet oxygen scavengers (tryptophan and ascorbic acid) and H2O2 on cell survival were evaluated. The morphology of bacteria after PDT was observed by transmission electron microscopy. Results: ICG-PDT inhibited the growth of bacteria by 5 log (99.999% inhibition) with 200 J/cm2 at 65.5 mW/cm2 in the presence of 100 μg/mL ICG. Adding 0.1% H2O2 at a lower PDT dose (25 μg/mL ICG and 100 J/cm2) increased its efficacy by 5 log. This PDT dose was not toxic to human fibroblasts. PDT significantly reduced the level of bacterial virulence factors. The inhibition effects were decreased by tryptophan and ascorbic acid suggested singlet oxygen involved in the process. TEM showed severe non-selective cell destruction immediately after irradiation. Conclusion: The study reveals ICG-PDT has the potential to treat MRSA by using a clinical accessible NIR lamp and photosensitizer.

原文English
頁(從 - 到)8-15
頁數8
期刊Dermatologica Sinica
36
發行號1
DOIs
出版狀態Published - 2018 三月 1

指紋

Indocyanine Green
Methicillin-Resistant Staphylococcus aureus
Light
Photochemotherapy
Singlet Oxygen
Photosensitizing Agents
Bacteria
Tryptophan
Ascorbic Acid
Fibroblasts
Poisons
Coagulase
Enterotoxins
Virulence Factors
Transmission Electron Microscopy
Bacterial Infections
Contrast Media
Virulence
1-phenyl-3,3-dimethyltriazene
Cell Survival

All Science Journal Classification (ASJC) codes

  • Dermatology

引用此文

@article{f4abae0c6c8049f49fee2b75660ec1ec,
title = "Photodynamic inactivation of methicillin-resistant Staphylococcus aureus by indocyanine green and near infrared light",
abstract = "Background: Antimicrobial photodynamic therapy (APDT) has become a potential regimen to treat multidrug-resistant bacterial infections. Limited data showed indocyanine green (ICG), a safe and inexpensive contrast medium for eye angiography and hepatic function examination, is an effective photosensitizer in APDT to kill methicillin-resistant Staphylococcus aureus (MRSA) after excitation with laser. Objective: We investigated the potentials of ICG-APDT with an inexpensive, non-coherent commercial near infrared (NIR) lamp against MRSA. Methods: The inhibition of MRSA was studied after exposing bacteria to NIR with different light doses and concentrations of ICG. The selectivity on MRSA was examined on human fibroblasts. Bacterial virulence including the activities of coagulase and enterotoxin was investigated. The effects of singlet oxygen scavengers (tryptophan and ascorbic acid) and H2O2 on cell survival were evaluated. The morphology of bacteria after PDT was observed by transmission electron microscopy. Results: ICG-PDT inhibited the growth of bacteria by 5 log (99.999{\%} inhibition) with 200 J/cm2 at 65.5 mW/cm2 in the presence of 100 μg/mL ICG. Adding 0.1{\%} H2O2 at a lower PDT dose (25 μg/mL ICG and 100 J/cm2) increased its efficacy by 5 log. This PDT dose was not toxic to human fibroblasts. PDT significantly reduced the level of bacterial virulence factors. The inhibition effects were decreased by tryptophan and ascorbic acid suggested singlet oxygen involved in the process. TEM showed severe non-selective cell destruction immediately after irradiation. Conclusion: The study reveals ICG-PDT has the potential to treat MRSA by using a clinical accessible NIR lamp and photosensitizer.",
author = "Tak-Wah Wong and Wu, {En Chi} and Wen-Chien Ko and Lee, {Ching Chi} and Hor, {Lien I.} and I-Hsiu Huang",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.dsi.2017.08.003",
language = "English",
volume = "36",
pages = "8--15",
journal = "Dermatologica Sinica",
issn = "1027-8117",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Photodynamic inactivation of methicillin-resistant Staphylococcus aureus by indocyanine green and near infrared light

AU - Wong, Tak-Wah

AU - Wu, En Chi

AU - Ko, Wen-Chien

AU - Lee, Ching Chi

AU - Hor, Lien I.

AU - Huang, I-Hsiu

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Background: Antimicrobial photodynamic therapy (APDT) has become a potential regimen to treat multidrug-resistant bacterial infections. Limited data showed indocyanine green (ICG), a safe and inexpensive contrast medium for eye angiography and hepatic function examination, is an effective photosensitizer in APDT to kill methicillin-resistant Staphylococcus aureus (MRSA) after excitation with laser. Objective: We investigated the potentials of ICG-APDT with an inexpensive, non-coherent commercial near infrared (NIR) lamp against MRSA. Methods: The inhibition of MRSA was studied after exposing bacteria to NIR with different light doses and concentrations of ICG. The selectivity on MRSA was examined on human fibroblasts. Bacterial virulence including the activities of coagulase and enterotoxin was investigated. The effects of singlet oxygen scavengers (tryptophan and ascorbic acid) and H2O2 on cell survival were evaluated. The morphology of bacteria after PDT was observed by transmission electron microscopy. Results: ICG-PDT inhibited the growth of bacteria by 5 log (99.999% inhibition) with 200 J/cm2 at 65.5 mW/cm2 in the presence of 100 μg/mL ICG. Adding 0.1% H2O2 at a lower PDT dose (25 μg/mL ICG and 100 J/cm2) increased its efficacy by 5 log. This PDT dose was not toxic to human fibroblasts. PDT significantly reduced the level of bacterial virulence factors. The inhibition effects were decreased by tryptophan and ascorbic acid suggested singlet oxygen involved in the process. TEM showed severe non-selective cell destruction immediately after irradiation. Conclusion: The study reveals ICG-PDT has the potential to treat MRSA by using a clinical accessible NIR lamp and photosensitizer.

AB - Background: Antimicrobial photodynamic therapy (APDT) has become a potential regimen to treat multidrug-resistant bacterial infections. Limited data showed indocyanine green (ICG), a safe and inexpensive contrast medium for eye angiography and hepatic function examination, is an effective photosensitizer in APDT to kill methicillin-resistant Staphylococcus aureus (MRSA) after excitation with laser. Objective: We investigated the potentials of ICG-APDT with an inexpensive, non-coherent commercial near infrared (NIR) lamp against MRSA. Methods: The inhibition of MRSA was studied after exposing bacteria to NIR with different light doses and concentrations of ICG. The selectivity on MRSA was examined on human fibroblasts. Bacterial virulence including the activities of coagulase and enterotoxin was investigated. The effects of singlet oxygen scavengers (tryptophan and ascorbic acid) and H2O2 on cell survival were evaluated. The morphology of bacteria after PDT was observed by transmission electron microscopy. Results: ICG-PDT inhibited the growth of bacteria by 5 log (99.999% inhibition) with 200 J/cm2 at 65.5 mW/cm2 in the presence of 100 μg/mL ICG. Adding 0.1% H2O2 at a lower PDT dose (25 μg/mL ICG and 100 J/cm2) increased its efficacy by 5 log. This PDT dose was not toxic to human fibroblasts. PDT significantly reduced the level of bacterial virulence factors. The inhibition effects were decreased by tryptophan and ascorbic acid suggested singlet oxygen involved in the process. TEM showed severe non-selective cell destruction immediately after irradiation. Conclusion: The study reveals ICG-PDT has the potential to treat MRSA by using a clinical accessible NIR lamp and photosensitizer.

UR - http://www.scopus.com/inward/record.url?scp=85029714571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85029714571&partnerID=8YFLogxK

U2 - 10.1016/j.dsi.2017.08.003

DO - 10.1016/j.dsi.2017.08.003

M3 - Article

VL - 36

SP - 8

EP - 15

JO - Dermatologica Sinica

JF - Dermatologica Sinica

SN - 1027-8117

IS - 1

ER -