Porous iron oxide based nanorods developed as delivery nanocapsules

Ping Ching Wu, Wen Shiuan Wang, Ying Ting Huang, Hwo Shuenn Sheu, Yi Wei Lo, Tsung Lin Tsai, Dar Bin Shieh, Chen Sheng Yeh

研究成果: Article

100 引文 (Scopus)

摘要

A low-temperature solution approach (90-95°C) using FeCl3 and urea was carried out to synthesize β-FeOOH nanorods in aqueous solution. The as-synthesized β-FeOOH nanorods were further calcined at 300°C to form porous nanorods with compositions including both β-FeOOH and α-Fe2O3. The derived porous nanorods were engineered to assemble with four layers of polyelectrolytes (polyacrylic acid (PAA)/polyethylenimine(PEI)/PAA/PEI) on their surfaces as polyelectrolyte multilayer nanocapsules. Fluorescein isothiocyanate (FITC) molecules were loaded into the polyelectrolyte multilayer nanocapsules in order to investigate drug release and intracellular delivery in Hela cells. The as-prepared nanocapsules showed ionic strength-dependent control of the permeability of the polyelectrolyte shells. The release behavior of the entrapped FITC from the FITC-loaded nanocapsules exhibited either controlled- or sustained-release trends, depending on the compactness of the polyelectrolyte shells on the nanorod surfaces. Cytotoxicity measurements demonstrate that the native nanorods and the polymer-coated nanorods have excellent bio-compatibility in ail dosages between 0.1 ng mL-1 and 100 μgm L-1. The time dependence of uptake of FITC-loaded nanocapsules by Hela cancer cells ob served by laser confocal microscopy in dicates that the nanocapsules can readily be taken up by cancer cells in 15 min, a relatively short period of time, while the slow release of the FITC from the initial perimembrane space into the cytoplasm was followed by release into the nucleus after 24 h.

原文English
頁(從 - 到)3878-3885
頁數8
期刊Chemistry - A European Journal
13
發行號14
DOIs
出版狀態Published - 2007 六月 21

指紋

Nanocapsules
Nanorods
Iron oxides
Polyelectrolytes
Fluorescein
carbopol 940
Polyethyleneimine
Multilayers
Cells
Acids
Confocal microscopy
Cytotoxicity
ferric oxide
Ionic strength
Biocompatibility
Urea
Polymers
isothiocyanic acid
Molecules
Lasers

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Organic Chemistry

引用此文

Wu, Ping Ching ; Wang, Wen Shiuan ; Huang, Ying Ting ; Sheu, Hwo Shuenn ; Lo, Yi Wei ; Tsai, Tsung Lin ; Shieh, Dar Bin ; Yeh, Chen Sheng. / Porous iron oxide based nanorods developed as delivery nanocapsules. 於: Chemistry - A European Journal. 2007 ; 卷 13, 編號 14. 頁 3878-3885.
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abstract = "A low-temperature solution approach (90-95°C) using FeCl3 and urea was carried out to synthesize β-FeOOH nanorods in aqueous solution. The as-synthesized β-FeOOH nanorods were further calcined at 300°C to form porous nanorods with compositions including both β-FeOOH and α-Fe2O3. The derived porous nanorods were engineered to assemble with four layers of polyelectrolytes (polyacrylic acid (PAA)/polyethylenimine(PEI)/PAA/PEI) on their surfaces as polyelectrolyte multilayer nanocapsules. Fluorescein isothiocyanate (FITC) molecules were loaded into the polyelectrolyte multilayer nanocapsules in order to investigate drug release and intracellular delivery in Hela cells. The as-prepared nanocapsules showed ionic strength-dependent control of the permeability of the polyelectrolyte shells. The release behavior of the entrapped FITC from the FITC-loaded nanocapsules exhibited either controlled- or sustained-release trends, depending on the compactness of the polyelectrolyte shells on the nanorod surfaces. Cytotoxicity measurements demonstrate that the native nanorods and the polymer-coated nanorods have excellent bio-compatibility in ail dosages between 0.1 ng mL-1 and 100 μgm L-1. The time dependence of uptake of FITC-loaded nanocapsules by Hela cancer cells ob served by laser confocal microscopy in dicates that the nanocapsules can readily be taken up by cancer cells in 15 min, a relatively short period of time, while the slow release of the FITC from the initial perimembrane space into the cytoplasm was followed by release into the nucleus after 24 h.",
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Porous iron oxide based nanorods developed as delivery nanocapsules. / Wu, Ping Ching; Wang, Wen Shiuan; Huang, Ying Ting; Sheu, Hwo Shuenn; Lo, Yi Wei; Tsai, Tsung Lin; Shieh, Dar Bin; Yeh, Chen Sheng.

於: Chemistry - A European Journal, 卷 13, 編號 14, 21.06.2007, p. 3878-3885.

研究成果: Article

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AU - Huang, Ying Ting

AU - Sheu, Hwo Shuenn

AU - Lo, Yi Wei

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AU - Shieh, Dar Bin

AU - Yeh, Chen Sheng

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AB - A low-temperature solution approach (90-95°C) using FeCl3 and urea was carried out to synthesize β-FeOOH nanorods in aqueous solution. The as-synthesized β-FeOOH nanorods were further calcined at 300°C to form porous nanorods with compositions including both β-FeOOH and α-Fe2O3. The derived porous nanorods were engineered to assemble with four layers of polyelectrolytes (polyacrylic acid (PAA)/polyethylenimine(PEI)/PAA/PEI) on their surfaces as polyelectrolyte multilayer nanocapsules. Fluorescein isothiocyanate (FITC) molecules were loaded into the polyelectrolyte multilayer nanocapsules in order to investigate drug release and intracellular delivery in Hela cells. The as-prepared nanocapsules showed ionic strength-dependent control of the permeability of the polyelectrolyte shells. The release behavior of the entrapped FITC from the FITC-loaded nanocapsules exhibited either controlled- or sustained-release trends, depending on the compactness of the polyelectrolyte shells on the nanorod surfaces. Cytotoxicity measurements demonstrate that the native nanorods and the polymer-coated nanorods have excellent bio-compatibility in ail dosages between 0.1 ng mL-1 and 100 μgm L-1. The time dependence of uptake of FITC-loaded nanocapsules by Hela cancer cells ob served by laser confocal microscopy in dicates that the nanocapsules can readily be taken up by cancer cells in 15 min, a relatively short period of time, while the slow release of the FITC from the initial perimembrane space into the cytoplasm was followed by release into the nucleus after 24 h.

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