Positively charged GFP-chromophore-based fluorescent sensor for DNA

Ming Ju Li; Jun Jia Xu; Robert Sung; Kuangsen Sung

doi:10.1016/j.optmat.2024.115581

Positively charged GFP-chromophore-based fluorescent sensor for DNA

Ming Ju Li, Jun Jia Xu, Robert Sung, Kuangsen Sung

化學系

研究成果: Article › 同行評審

1 引文斯高帕斯（Scopus）

摘要

Fluorescence titration of the positively charged green fluorescent protein (GFP) chromophore analogue 1 with E. Coli plasmid DNA [pcDNA3.1(−)] confirms that 1 serves as a fluorescent probe for sensing E. Coli plasmid DNA. The binding constant K between the DNA and 1 is as large as 2.9 × 10⁵. The sensing mechanism is that the intermolecular steric hindrance generated between 1 and E. Coli plasmid DNA by electrostatic attraction restricts the φ-torsion around P-bond and τ-torsion around I-bond of 1, enhancing fluorescence of 1.

原文	English
文章編號	115581
期刊	Optical Materials
卷	153
DOIs	https://doi.org/10.1016/j.optmat.2024.115581
出版狀態	Published - 2024 7月

All Science Journal Classification (ASJC) codes

電子、光磁材料
原子與分子物理與光學
光譜
物理與理論化學
有機化學
無機化學
電氣與電子工程

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10.1016/j.optmat.2024.115581

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title = "Positively charged GFP-chromophore-based fluorescent sensor for DNA",

abstract = "Fluorescence titration of the positively charged green fluorescent protein (GFP) chromophore analogue 1 with E. Coli plasmid DNA [pcDNA3.1(−)] confirms that 1 serves as a fluorescent probe for sensing E. Coli plasmid DNA. The binding constant K between the DNA and 1 is as large as 2.9 × 105. The sensing mechanism is that the intermolecular steric hindrance generated between 1 and E. Coli plasmid DNA by electrostatic attraction restricts the φ-torsion around P-bond and τ-torsion around I-bond of 1, enhancing fluorescence of 1.",

author = "Li, {Ming Ju} and Xu, {Jun Jia} and Robert Sung and Kuangsen Sung",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2024",

month = jul,

doi = "10.1016/j.optmat.2024.115581",

language = "English",

volume = "153",

journal = "Optical Materials",

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TY - JOUR

T1 - Positively charged GFP-chromophore-based fluorescent sensor for DNA

AU - Li, Ming Ju

AU - Xu, Jun Jia

AU - Sung, Robert

AU - Sung, Kuangsen

PY - 2024/7

Y1 - 2024/7

N2 - Fluorescence titration of the positively charged green fluorescent protein (GFP) chromophore analogue 1 with E. Coli plasmid DNA [pcDNA3.1(−)] confirms that 1 serves as a fluorescent probe for sensing E. Coli plasmid DNA. The binding constant K between the DNA and 1 is as large as 2.9 × 105. The sensing mechanism is that the intermolecular steric hindrance generated between 1 and E. Coli plasmid DNA by electrostatic attraction restricts the φ-torsion around P-bond and τ-torsion around I-bond of 1, enhancing fluorescence of 1.

AB - Fluorescence titration of the positively charged green fluorescent protein (GFP) chromophore analogue 1 with E. Coli plasmid DNA [pcDNA3.1(−)] confirms that 1 serves as a fluorescent probe for sensing E. Coli plasmid DNA. The binding constant K between the DNA and 1 is as large as 2.9 × 105. The sensing mechanism is that the intermolecular steric hindrance generated between 1 and E. Coli plasmid DNA by electrostatic attraction restricts the φ-torsion around P-bond and τ-torsion around I-bond of 1, enhancing fluorescence of 1.

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DO - 10.1016/j.optmat.2024.115581

M3 - Article

AN - SCOPUS:85194081321

SN - 0925-3467

VL - 153

JO - Optical Materials

JF - Optical Materials

M1 - 115581

ER -

Positively charged GFP-chromophore-based fluorescent sensor for DNA

摘要

All Science Journal Classification (ASJC) codes

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