Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles

Hsueh Cheng Chiang, Wonchul Shin, Wei Dong Zhao, Edaeni Hamid, Jiansong Sheng, Maryna Baydyuk, Peter J. Wen, Albert Jin, Fanny Momboisse, Ling Gang Wu

研究成果: Article同行評審

47 引文 斯高帕斯(Scopus)

摘要

Vesicle fusion with the plasma membrane generates an Ω-shaped membrane profile. Its pore is thought to dilate until flattening (full-collapse), followed by classical endocytosis to retrieve vesicles. Alternatively, the pore may close (kiss-and-run), but the triggering mechanisms and its endocytic roles remain poorly understood. Here, using confocal and stimulated emission depletion microscopy imaging of dense-core vesicles, we find that fusion-generated Ω-profiles may enlarge or shrink while maintaining vesicular membrane proteins. Closure of fusion-generated Ω-profiles, which produces various sizes of vesicles, is the dominant mechanism mediating rapid and slow endocytosis within ∼1-30 s. Strong calcium influx triggers dynamin-mediated closure. Weak calcium influx does not promote closure, but facilitates the merging of Ω-profiles with the plasma membrane via shrinking rather than full-collapse. These results establish a model, termed Ω-exo-endocytosis, in which the fusion-generated Ω-profile may shrink to merge with the plasma membrane, change in size or change in size then close in response to calcium, which is the main mechanism to retrieve dense-core vesicles.

原文English
文章編號3356
期刊Nature communications
5
DOIs
出版狀態Published - 2014 二月 24

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 生物化學、遺傳與分子生物學 (全部)
  • 物理與天文學 (全部)

指紋

深入研究「Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles」主題。共同形成了獨特的指紋。

引用此