Potential of a simple solid-phase extraction method coupled to analytical and bioanalytical methods for an improved determination of microcystins in algal samples

Yi Min Chen, Tzong Huei Lee, Shyh Jye Lee, Jian Zhi Lin, Rang Huang, Hong Nong Chou

研究成果: Article同行評審

6 引文 斯高帕斯(Scopus)

摘要

Artemia assays and protein phosphatase assays are commonly used for the screening of microcystins (MCs) in algal samples instead of the standard mouse toxicity assay. However, it has been shown that their results are often biased because of the matrix effects. To eliminate the possible interferences in the algal matrices, a new solid-phase extraction (SPE) method using silica gel as a sorbent was developed and evaluated. Results show that this SPE method could not only reduce the toxicity of the Microcystis samples towards brine shrimp by 50-80% but also eliminate 90-100% of the endogenous phosphatase activity from Spirulina and Chlorella samples, thus improving the determination of microcystins in algal samples using either of the two bioanalytical methods. The application of this SPE method as an off-line cleanup for high-performance liquid chromatography (HPLC) with UV detection is also described in this study. After SPE, the HPLC chromatograms of Microcystis samples have clear baselines that have no interferences with the analyte peaks.

原文English
頁(從 - 到)134-141
頁數8
期刊Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
844
發行號1
DOIs
出版狀態Published - 2006 十一月 21

All Science Journal Classification (ASJC) codes

  • 分析化學
  • 生物化學
  • 臨床生物化學
  • 細胞生物學

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