Potential of Autologous Progenitor Cells and Decellularized Porcine Artery Matrix in Construction of Tissue-engineered Vascular Grafts

Jieh Neng Wang, Chung Dann Kan, Shao Hsien Lin, Ko Chi Chang, Stephanie Tsao, Tak Wah Wong

研究成果: Article同行評審

4 引文 斯高帕斯(Scopus)

摘要

To develop a tissue-engineered vascular graft, we used pericardial effusion-derived progenitor cells (PEPCs) collected from drained fluid after open-heart surgery in children with congenital heart diseases to repopulate a decellularized porcine pulmonary artery. The PEPCs were compared with human fibroblasts (HS68) and human umbilical vein endothelial cells (HUVECs) in cell growth and migration. They were cultured with the matrices via an inner approach (intima), lateral approach (media), and outer approach (adventitia). PEPCs grew and migrated better than the other two cells 14 days after seeding in the decellularized vessel. In immunofluorescence assays, PEPCs expressed CD90 and CD105 indicating a vascular differentiation. PEPCs grew in a decellularized porcine pulmonary artery matrix may have the potential for producing tissue-engineered vascular grafts.

原文English
頁(從 - 到)72-84
頁數13
期刊Organogenesis
17
發行號3-4
DOIs
出版狀態Published - 2021

All Science Journal Classification (ASJC) codes

  • 胚胎學
  • 生物醫學工程
  • 發展生物學
  • 移植

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