Practical synthesis of maleimides and coumarin-linked probes for protein and antibody labelling via reduction of native disulfides

Hong Y. Song, Mun H. Ngai, Zhen Y. Song, Paul A. MacAry, Jonathan Hobley, Martin J. Lear

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72 引文 斯高帕斯(Scopus)

摘要

The cellular tracking, detection and sensing of protein or antibody movement are important aspects to advance our understanding of biomolecular interactions and activity. Antibodies modified with fluorescent dyes are also valuable tools, especially in immunology research. We describe here a proof-of-principle study of a new water-soluble coumarin probe with a maleimide thiol-reacting unit to fluorescently tag biomolecules. Highlights include: (1) a convenient water-based preparation of N-substituted maleimides, (2) a one-pot preparation of activated maleimido-esters, and (3) a bio-conjugation protocol for the selenol-promoted reduction of native disulfide bonds and the 'site-specific' labelling of antibodies with no significant loss of activity.

原文English
頁(從 - 到)3400-3406
頁數7
期刊Organic and Biomolecular Chemistry
7
發行號17
DOIs
出版狀態Published - 2009

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 物理與理論化學
  • 有機化學

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