Precise control of microtubule disassembly in living cells

Grace Y. Liu, Shiau Chi Chen, Gang Hui Lee, Kritika Shaiv, Pin Yu Chen, Hsuan Cheng, Shi Rong Hong, Wen Ting Yang, Shih Han Huang, Ya Chu Chang, Hsien Chu Wang, Ching Lin Kao, Pin Chiao Sun, Ming Hong Chao, Yian Ying Lee, Ming Jer Tang, Yu Chun Lin

研究成果: Article同行評審

13 引文 斯高帕斯(Scopus)

摘要

Microtubules tightly regulate various cellular activities. Our understanding of microtubules is largely based on experiments using microtubule-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific microtubule populations, due to their slow effects on the entire pool of microtubules. To overcome this technological limitation, we have used chemo and optogenetics to disassemble specific microtubule subtypes, including tyrosinated microtubules, primary cilia, mitotic spindles, and intercellular bridges, by rapidly recruiting engineered microtubule-cleaving enzymes onto target microtubules in a reversible manner. Using this approach, we show that acute microtubule disassembly swiftly halts vesicular trafficking and lysosomal dynamics. It also immediately triggers Golgi and ER reorganization and slows the fusion/fission of mitochondria without affecting mitochondrial membrane potential. In addition, cell rigidity is increased after microtubule disruption owing to increased contractile stress fibers. Microtubule disruption furthermore prevents cell division, but does not cause cell death during interphase. Overall, the reported tools facilitate detailed analysis of how microtubules precisely regulate cellular architecture and functions.

原文English
文章編號e110472
期刊EMBO Journal
41
發行號15
DOIs
出版狀態Published - 2022 8月 1

All Science Journal Classification (ASJC) codes

  • 一般神經科學
  • 分子生物學
  • 一般生物化學,遺傳學和分子生物學
  • 一般免疫學和微生物學

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