Predicting and testing physical locations of genetically mapped loci on tomato pachytene chromosome 1

Song Bin Chang, Lorinda K. Anderson, Jamie D. Sherman, Suzanne M. Royer, Stephen M. Stack

研究成果: Article同行評審

30 引文 斯高帕斯(Scopus)

摘要

Predicting the chromosomal location of mapped markers has been difficult because linkage maps do not reveal differences in crossover frequencies along the physical structure of chromosomes. Here we combine a physical crossover map based on the distribution of recombination nodules (RNs) on Solanum lycopersicum (tomato) synaptonemal complex 1 with a molecular genetic linkage map from the interspecific hybrid S. lycopersicum X S. pennellii to predict the physical locations of 17 mapped loci on tomato pachytene chromosome 1. Except for one marker located in heterochromatin, the predicted locations agree well with the observed locations determined by fluorescence in situ hybridization. One advantage of this approach is that once the RN distribution has been determined, the chromosomal location of any mapped locus (current or future) can be predicted with a high level of confidence.

原文English
頁(從 - 到)2131-2138
頁數8
期刊Genetics
176
發行號4
DOIs
出版狀態Published - 2007 八月

All Science Journal Classification (ASJC) codes

  • 遺傳學

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