Predominance of methicillin-resistant Staphylococcus aureus in the residents and environments of long-term care facilities in Taiwan

Infection Control Society of Taiwan

研究成果: Article

3 引文 (Scopus)

摘要

Background/purpose: This study investigated the distribution and persistence of multidrug resistant organisms (MDROs) including methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant Enterobacteriaceae (CRE), carbapenem-resistant Pseudomonas aeruginosa (CRPA), and multidrug-resistant Acinetobacter baumannii (MDRAB) in six long-term care facilities (LTCFs). Methods: We investigated the distribution of MDROs in residents of six LTCFs and their environments from January to December 2016 (intervention period). Active surveillance of colonization of MDROs was performed by culturing rectal and nasal swab samples from the residents every three months. Multilocus sequence typing (MLST) was conducted, and genes for panton-valentine leukocidin (PVL) from MRSA isolates were determined. Results: A total of 521 samples were positive for MDROs, and MRSA was the most common organism (65.1%), followed by MDRAB (11.3%), carbapenem-resistant Klebsiella pneumoniae (11.1%), carbapenem-resistant Escherichia coli (4.6%), and carbapenem-resistant P. aeruginosa (2.1%, n = 11). By a linear regression model, positive MRSA isolates from the environment were found to be statistically significant and associated with the number of colonized LTCF residents (p = 0.01), while the timing of the surveillance culture was not (p = 0.227). The main MLST types associated with PVL-production were sequence type (ST) 59, (40.0%, 24/60), ST30 (21.4%, 3/14), ST8 (87.5%, 14/16), and ST45 (3.6%, 1/28). The susceptibility rates of tetracycline (96.7%), trimethoprim-sulfamethoxazole (96.7%), and ciprofloxacin (81.7%) were statistically significant and higher in MRSA ST59, compared to the rates in MRSA ST45 isolates. Conclusions: MRSA was the most commonly colonized MDRO, both in the LTCF residents and in the environment, followed by MDRAB and carbapenem-resistant K. pneumoniae.

原文English
頁(從 - 到)62-74
頁數13
期刊Journal of Microbiology, Immunology and Infection
52
發行號1
DOIs
出版狀態Published - 2019 二月 1

指紋

Long-Term Care
Methicillin-Resistant Staphylococcus aureus
Carbapenems
Taiwan
Acinetobacter baumannii
Multilocus Sequence Typing
Klebsiella pneumoniae
Pseudomonas aeruginosa
Linear Models
Sulfamethoxazole Drug Combination Trimethoprim
Enterobacteriaceae
Ciprofloxacin
Tetracycline
Nose
Escherichia coli
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

引用此文

@article{cb6ad52676bb4c7fa160fc1d0c1ae7cc,
title = "Predominance of methicillin-resistant Staphylococcus aureus in the residents and environments of long-term care facilities in Taiwan",
abstract = "Background/purpose: This study investigated the distribution and persistence of multidrug resistant organisms (MDROs) including methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant Enterobacteriaceae (CRE), carbapenem-resistant Pseudomonas aeruginosa (CRPA), and multidrug-resistant Acinetobacter baumannii (MDRAB) in six long-term care facilities (LTCFs). Methods: We investigated the distribution of MDROs in residents of six LTCFs and their environments from January to December 2016 (intervention period). Active surveillance of colonization of MDROs was performed by culturing rectal and nasal swab samples from the residents every three months. Multilocus sequence typing (MLST) was conducted, and genes for panton-valentine leukocidin (PVL) from MRSA isolates were determined. Results: A total of 521 samples were positive for MDROs, and MRSA was the most common organism (65.1{\%}), followed by MDRAB (11.3{\%}), carbapenem-resistant Klebsiella pneumoniae (11.1{\%}), carbapenem-resistant Escherichia coli (4.6{\%}), and carbapenem-resistant P. aeruginosa (2.1{\%}, n = 11). By a linear regression model, positive MRSA isolates from the environment were found to be statistically significant and associated with the number of colonized LTCF residents (p = 0.01), while the timing of the surveillance culture was not (p = 0.227). The main MLST types associated with PVL-production were sequence type (ST) 59, (40.0{\%}, 24/60), ST30 (21.4{\%}, 3/14), ST8 (87.5{\%}, 14/16), and ST45 (3.6{\%}, 1/28). The susceptibility rates of tetracycline (96.7{\%}), trimethoprim-sulfamethoxazole (96.7{\%}), and ciprofloxacin (81.7{\%}) were statistically significant and higher in MRSA ST59, compared to the rates in MRSA ST45 isolates. Conclusions: MRSA was the most commonly colonized MDRO, both in the LTCF residents and in the environment, followed by MDRAB and carbapenem-resistant K. pneumoniae.",
author = "{Infection Control Society of Taiwan} and Liu, {Chia Ying} and Lai, {Chih Cheng} and Chiang, {Hsiu Tzy} and Lu, {Min Chi} and Wang, {Ling Fang} and Tsai, {Tsai Ling} and Kang, {Mei Yu} and Jan, {Yi Ni} and Lo, {Yi Ting} and Wen-Chien Ko and Tseng, {Shu Hui} and Lee, {Chun Ming} and Hsueh, {Po Ren}",
year = "2019",
month = "2",
day = "1",
doi = "10.1016/j.jmii.2018.02.001",
language = "English",
volume = "52",
pages = "62--74",
journal = "Journal of Microbiology, Immunology and Infection",
issn = "1684-1182",
publisher = "Elsevier Taiwan LLC",
number = "1",

}

TY - JOUR

T1 - Predominance of methicillin-resistant Staphylococcus aureus in the residents and environments of long-term care facilities in Taiwan

AU - Infection Control Society of Taiwan

AU - Liu, Chia Ying

AU - Lai, Chih Cheng

AU - Chiang, Hsiu Tzy

AU - Lu, Min Chi

AU - Wang, Ling Fang

AU - Tsai, Tsai Ling

AU - Kang, Mei Yu

AU - Jan, Yi Ni

AU - Lo, Yi Ting

AU - Ko, Wen-Chien

AU - Tseng, Shu Hui

AU - Lee, Chun Ming

AU - Hsueh, Po Ren

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Background/purpose: This study investigated the distribution and persistence of multidrug resistant organisms (MDROs) including methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant Enterobacteriaceae (CRE), carbapenem-resistant Pseudomonas aeruginosa (CRPA), and multidrug-resistant Acinetobacter baumannii (MDRAB) in six long-term care facilities (LTCFs). Methods: We investigated the distribution of MDROs in residents of six LTCFs and their environments from January to December 2016 (intervention period). Active surveillance of colonization of MDROs was performed by culturing rectal and nasal swab samples from the residents every three months. Multilocus sequence typing (MLST) was conducted, and genes for panton-valentine leukocidin (PVL) from MRSA isolates were determined. Results: A total of 521 samples were positive for MDROs, and MRSA was the most common organism (65.1%), followed by MDRAB (11.3%), carbapenem-resistant Klebsiella pneumoniae (11.1%), carbapenem-resistant Escherichia coli (4.6%), and carbapenem-resistant P. aeruginosa (2.1%, n = 11). By a linear regression model, positive MRSA isolates from the environment were found to be statistically significant and associated with the number of colonized LTCF residents (p = 0.01), while the timing of the surveillance culture was not (p = 0.227). The main MLST types associated with PVL-production were sequence type (ST) 59, (40.0%, 24/60), ST30 (21.4%, 3/14), ST8 (87.5%, 14/16), and ST45 (3.6%, 1/28). The susceptibility rates of tetracycline (96.7%), trimethoprim-sulfamethoxazole (96.7%), and ciprofloxacin (81.7%) were statistically significant and higher in MRSA ST59, compared to the rates in MRSA ST45 isolates. Conclusions: MRSA was the most commonly colonized MDRO, both in the LTCF residents and in the environment, followed by MDRAB and carbapenem-resistant K. pneumoniae.

AB - Background/purpose: This study investigated the distribution and persistence of multidrug resistant organisms (MDROs) including methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant Enterobacteriaceae (CRE), carbapenem-resistant Pseudomonas aeruginosa (CRPA), and multidrug-resistant Acinetobacter baumannii (MDRAB) in six long-term care facilities (LTCFs). Methods: We investigated the distribution of MDROs in residents of six LTCFs and their environments from January to December 2016 (intervention period). Active surveillance of colonization of MDROs was performed by culturing rectal and nasal swab samples from the residents every three months. Multilocus sequence typing (MLST) was conducted, and genes for panton-valentine leukocidin (PVL) from MRSA isolates were determined. Results: A total of 521 samples were positive for MDROs, and MRSA was the most common organism (65.1%), followed by MDRAB (11.3%), carbapenem-resistant Klebsiella pneumoniae (11.1%), carbapenem-resistant Escherichia coli (4.6%), and carbapenem-resistant P. aeruginosa (2.1%, n = 11). By a linear regression model, positive MRSA isolates from the environment were found to be statistically significant and associated with the number of colonized LTCF residents (p = 0.01), while the timing of the surveillance culture was not (p = 0.227). The main MLST types associated with PVL-production were sequence type (ST) 59, (40.0%, 24/60), ST30 (21.4%, 3/14), ST8 (87.5%, 14/16), and ST45 (3.6%, 1/28). The susceptibility rates of tetracycline (96.7%), trimethoprim-sulfamethoxazole (96.7%), and ciprofloxacin (81.7%) were statistically significant and higher in MRSA ST59, compared to the rates in MRSA ST45 isolates. Conclusions: MRSA was the most commonly colonized MDRO, both in the LTCF residents and in the environment, followed by MDRAB and carbapenem-resistant K. pneumoniae.

UR - http://www.scopus.com/inward/record.url?scp=85043305661&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85043305661&partnerID=8YFLogxK

U2 - 10.1016/j.jmii.2018.02.001

DO - 10.1016/j.jmii.2018.02.001

M3 - Article

C2 - 29530709

AN - SCOPUS:85043305661

VL - 52

SP - 62

EP - 74

JO - Journal of Microbiology, Immunology and Infection

JF - Journal of Microbiology, Immunology and Infection

SN - 1684-1182

IS - 1

ER -