Preparation and cell compatibility evaluation of chitosan/collagen composite scaffolds using amino acids as crosslinking bridges

Sung Pei Tsai, Chien Yang Hsieh, Chung Yu Hsieh, Da Ming Wang, Lynn Ling Huei Huang, Juin Yih Lai, Hsyue Jen Hsieh

研究成果: Article同行評審

56 引文 斯高帕斯(Scopus)

摘要

In this study, a novel freeze-gelation method instead of the conventional freeze-drying method was used to fabricate porous chitosan/collagen-based composite scaffolds for skin-related tissue engineering applications. To improve the performance of chitosan/collagen composite scaffolds, we added 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and amino acids (including alanine, glycine, and glutamic acid) in the fabrication procedure of the composite scaffolds, in which amino acid molecules act as crosslinking bridges to enhance the EDC-mediated crosslinking. This novel combination enhanced the tensile strength of the scaffolds from 0.70 N/g for uncrosslinked scaffolds to 2.2 N/g for crosslinked ones; the crosslinked scaffolds also exhibited slower degradation rates. The hydrophilicity of the scaffolds was also significantly enhanced by the addition of amino acids to the scaffolds. Cell compatibility was demonstrated by the in vitro culture of human skin fibroblasts on the scaffolds. The fibroblasts attached and proliferated well on the chitosan/collagen composite scaffolds, especially the one with glutamic acid molecules as crosslinking bridges, whereas cells did not grow on the chitosan scaffolds. Our results suggest that the collagen-modified chitosan scaffolds with glutamic acid molecules as crosslinking bridges are very promising biomaterials for skin-related tissue engineering applications because of their enhanced tensile strength and improved cell compatibility with skin fibroblasts.

原文English
頁(從 - 到)1774-1785
頁數12
期刊Journal of Applied Polymer Science
105
發行號4
DOIs
出版狀態Published - 2007 八月 15

All Science Journal Classification (ASJC) codes

  • 化學 (全部)
  • 表面、塗料和薄膜
  • 聚合物和塑料
  • 材料化學

指紋

深入研究「Preparation and cell compatibility evaluation of chitosan/collagen composite scaffolds using amino acids as crosslinking bridges」主題。共同形成了獨特的指紋。

引用此