Preparation and characterization of a penicillin electrode based on crosslinked PVA membrane

C. B. Wu, S. M. Kuo, S. J. Chang, Y. J. Wang, Jui-Hsiang Liu

研究成果: Article

5 引文 斯高帕斯(Scopus)

摘要

Poly(vinyl alcohol), PVA, membrane was prepared by crosslinking reaction initiated by UV irradiation in the presence of sodium benzoate and benzoic acid. Penicillinase was immobilized after the PVA membrane had been activated with cyanuric chloride. The penicillinase-immobilized PVA membrane was then coupled with a pH glass electrode and used as a penicillin sensor. The effective diffusion coefficient of hydrogen ions through the penicillinase-immobilized PVA membrane was determined to be about 1.4×10-6 cm2/sec. This value is about two orders of magnitude higher than that obtained from the penicillinase-BSA (bovine serum albumin) membrane. Increasing the buffer strength increased the mass transfer rate of hydrogen ions, but decreased the sensitivity of the enzyme electrode. The higher buffer strength nevertheless increased the linear analytical range of the penicillin electrode. These results demonstrate the applicability of using PVA hydrogel as an excellent enzyme immobilized matrix for biosensor.

原文English
頁(從 - 到)435-440
頁數6
期刊Journal of the Chinese Institute of Chemical Engineers
28
發行號6
出版狀態Published - 1997 十一月 1

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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