Presence of DAZL transcript and protein in mature human spermatozoa

YungMing Lin, Chaio Wei Chen, Hsiao-Fang Sun, Shaw-Jenq Tsai, Johnny Shinn Nan Lin, Pao-Lin Kuo

研究成果: Article

24 引文 斯高帕斯(Scopus)

摘要

Objective: To identify the DAZL transcript and protein location in human spermatozoa. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): A fertile volunteer. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunostaining for DAZL. Main Outcome Measure(s): Expression of DAZL in human spermatozoa. Result(s): The DAZL-specific primers yield a 128 bp product in ejaculate. A protein of approximately 33.5 kDa was detected by Western blot analysis. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa and weak staining in the principal piece. A speckled-type distribution was found in the head region. Conclusion(s): The DAZL transcript and protein are present in human spermatozoa. The roles of DAZL protein in sperm motility and in the sperm-oocyte interaction await further investigation.

原文English
頁(從 - 到)626-629
頁數4
期刊Fertility and Sterility
77
發行號3
DOIs
出版狀態Published - 2002 三月 9

    指紋

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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