摘要
Objective: To identify the DAZL transcript and protein location in human spermatozoa. Design: In vitro experiment. Setting: University-based reproductive genetics laboratory. Patient(s): A fertile volunteer. Intervention(s): Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and immunostaining for DAZL. Main Outcome Measure(s): Expression of DAZL in human spermatozoa. Result(s): The DAZL-specific primers yield a 128 bp product in ejaculate. A protein of approximately 33.5 kDa was detected by Western blot analysis. Immunofluorescence staining showed strong homogeneous staining in the midpiece of spermatozoa and weak staining in the principal piece. A speckled-type distribution was found in the head region. Conclusion(s): The DAZL transcript and protein are present in human spermatozoa. The roles of DAZL protein in sperm motility and in the sperm-oocyte interaction await further investigation.
原文 | English |
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頁(從 - 到) | 626-629 |
頁數 | 4 |
期刊 | Fertility and Sterility |
卷 | 77 |
發行號 | 3 |
DOIs | |
出版狀態 | Published - 2002 |
All Science Journal Classification (ASJC) codes
- 生殖醫學
- 婦產科