TY - JOUR
T1 - Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits
AU - Olivos-Meza, A.
AU - Fitzsimmons, J. S.
AU - Casper, M. E.
AU - Chen, Q.
AU - An, K. N.
AU - Ruesink, T. J.
AU - O'Driscoll, S. W.
AU - Reinholz, G. G.
N1 - Funding Information:
Role of the funding source: Funding for this project was provided by National Institutes of Health (NIH) and National Institute of Arthritis and Musculoskeletal Diseases (NIAMS) through grant AR52115 and supplements to this grant for M.E. Casper. Dr Olivos-Meza also received support from the Government of Veracruz State, and the University of Veracruz, Mexico. Mayo Clinic and the Department of Orthopedic Surgery provided additional funding support for this project. These funding sources were not involved in any other aspect of the study.
PY - 2010/9
Y1 - 2010/9
N2 - Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.
AB - Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.
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U2 - 10.1016/j.joca.2010.06.003
DO - 10.1016/j.joca.2010.06.003
M3 - Article
C2 - 20633683
AN - SCOPUS:77956010301
SN - 1063-4584
VL - 18
SP - 1183
EP - 1191
JO - Osteoarthritis and Cartilage
JF - Osteoarthritis and Cartilage
IS - 9
ER -