TY - JOUR
T1 - Production, extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1
AU - Chen, Chun Yen
AU - Jesisca,
AU - Hsieh, Chienyan
AU - Lee, Duu Jong
AU - Chang, Chien Hsiang
AU - Chang, Jo Shu
N1 - Funding Information:
The authors also gratefully acknowledge the support received from Taiwan’s Ministry of Science and Technology under Grant Numbers MOST104-3113-E-006-003 and 103-2221-E-006-190-MY3. This work was also supported by Ministry of Education under the Top University Grants (also known as the “5-Years-50-Billion” Grants).
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21. mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20. min) and 99.5% (40. min) at 850. mbar and 25. °C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450. mbar, 35. °C and 40. min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8. days, respectively.
AB - The efficiencies of extraction and preservation of lutein from microalgae are critical for the success of its commercialization. In this study, lutein was produced by Chlorella sorokiniana MB-1 via semi-batch mixotrophic cultivation. The microalgal biomass with a lutein content of 5.21. mg/g was pretreated by bead-beating and high pressure cell disruption methods, and the lutein content was harvested by a reduced pressure extraction method. The effect of pretreatment, pressure, solvent type, extraction time and temperature on lutein recovery was investigated. Using high pressure pretreatment followed by extraction with tetrahydrofuran (THF) as solvent resulted in high lutein recovery efficiencies of 87.0% (20. min) and 99.5% (40. min) at 850. mbar and 25. °C. In contrast, using ethanol as the solvent, 86.2% lutein recovery was achieved under 450. mbar, 35. °C and 40. min extraction. The extracted lutein was stabilized in olive oil or sunflower oil with half-lives of 53.1 and 63.8. days, respectively.
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U2 - 10.1016/j.biortech.2015.10.071
DO - 10.1016/j.biortech.2015.10.071
M3 - Article
C2 - 26519703
AN - SCOPUS:84945378692
SN - 0960-8524
VL - 200
SP - 500
EP - 505
JO - Bioresource technology
JF - Bioresource technology
ER -