Progressive renal distortion by multiple cysts in transgenic mice expressing artificial micrornas against PKD1

Ellian Wang, Hsiu Mei Hsieh-Li, Yuan Yow Chiou, Yi Lin Chien, Hua Hui Ho, Hsian Jean Chin, Chi Kuang Leo Wang, San Chi Liang, Si Tse Jiang

研究成果: Article同行評審

27 引文 斯高帕斯(Scopus)

摘要

Autosomal dominant polycystic kidney disease (ADPKD) is one of the most common life-threatening inherited diseases, and the PKD1 gene is responsible for most cases of this disease. Previous efforts to establish a mouse model that recapitulates the phenotypic characteristics of ADPKD, which have used conventional or conditional knockout of the mouse orthologue Pkd1, have been unsuccessful or unreliable. In a previous study, we described the generation of a novel Pkd1 hypomorphic allele, in which Pkd1 expression was significantly reduced but not totally blocked. These Pkd1 homozygous mutant mice rapidly developed renal cystic disease, supporting the hypothesis that 'haploinsufficiency' explains development of the ADPKD phenotype. In the present study, we further investigated the Pkd1 haploinsufficiency effect by generating Pkd1 knockdown transgenic mice with co-cistronic expression of two miRNA hairpins specific to Pkd1 transcript and an Emerald GFP reporter driven by a human ubiquitin B promoter. Two transgenic lines which had ∼60-70% reduction of Pkd1 expression developed severe renal cystic disease at a rate similar to that of human ADPKD. These results further support the haploinsufficiency hypothesis, and suggest that the onset and progression of the renal cystic diseases are correlated with the level of Pkd1 expression. The two novel mutant lines of mice appear to be ideal models for the study of ADPKD.

原文English
頁(從 - 到)238-248
頁數11
期刊Journal of Pathology
222
發行號3
DOIs
出版狀態Published - 2010 十一月

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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