Prolonged use of high-dose morphine impairs angiogenesis and mobilization of endothelial progenitor cells in mice

Chen Fuh Lam, Pei Jung Chang, Yu Sheng Huang, Yen Hui Sung, Chien Chi Huang, Ming Wei Lin, Yen Chin Liu, Yu Chuan Tsai

研究成果: Article同行評審

56 引文 斯高帕斯(Scopus)

摘要

BACKGROUND: Morphine is one of the most commonly prescribed analgesics for treating wound pain. Using a mouse model of excisional wound injury, we determined the effects of high-dose morphine on angiogenesis and mobilization of endothelial progenitor cells. METHODS: An excisional wound was created on mice treated with placebo or morphine (20 mg/kg, i.p. injection for 14 days). Wound healing was compared by measuring the final-to-initial wound area ratio. Generation of superoxide anions in the wound was determined by luminol-enhanced chemiluminescence. Circulating mononuclear cells were isolated and measured for endothelial progenitor cell (defined as CD34+/CD133+ cell) counts. In vivo and in vitro measurements of angiogenesis after morphine treatment were performed using the Matrigel assay. RESULTS: Mice treated with morphine had reduced wound closure and higher wound superoxide ions concentrations than control mice. Morphine reduced the number of postwound circulating endothelial progenitor cells. Matrigel assay showed impaired angiogenesis in animals and reduced capillary tube formation in cultured endothelial cells treated with morphine. CONCLUSION: High-dose morphine impaired angiogenesis, increased systemic oxidative stress, and impaired mobilization of endothelial progenitor cells. This study emphasizes the potential detrimental effect of high-dose morphine on angiogenesis after systemic administration.

原文English
頁(從 - 到)686-692
頁數7
期刊Anesthesia and analgesia
107
發行號2
DOIs
出版狀態Published - 2008 八月

All Science Journal Classification (ASJC) codes

  • 麻醉與疼痛醫學

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