Prostate cancer-specific thermo-responsive polymer-coated iron oxide nanoparticles

Aniket S. Wadajkar, Jyothi U. Menon, Yuh Shyan Tsai, Crystal Gore, Timothy Dobin, Leah Gandee, Kim Kangasniemi, Masaya Takahashi, Bikash Manandhar, Jung Mo Ahn, Jer Tsong Hsieh, Kytai T. Nguyen

研究成果: Article同行評審

70 引文 斯高帕斯(Scopus)

摘要

Thermo-responsive poly(N-isopropylacrylamide-acrylamide-allylamine)-coated magnetic nanoparticles (PMNPs) were developed and conjugated with prostate cancer-specific R11 peptides for active targeting and imaging of prostate cancer. The stable nanoparticles with an average diameter of 100 nm and surface charge of -27.0 mV, had a lower critical solution temperature of 40 °C. Magnetic characterization showed that the nanoparticles can be recruited using a magnetic field and possess superparamagnetic behavior even after R11 conjugation. In vitro cell studies demonstrated that R11-conjugated PMNPs (R11-PMNPs) were compatible with human dermal fibroblasts and normal prostate epithelial cells to all tested concentrations up to 500 μg/ml after 24 h of incubation. Moreover, the nanoparticles were taken up by prostate cancer cells (PC3 and LNCaP) in a dose-dependent manner, which was higher in case of R11-PMNPs than PMNPs. Further, in vivo biodistribution of the nanoparticles showed significantly more R11-PMNPs accumulation in tumors than other vital organs unlike PMNPs without R11 conjugation. Moreover, R11-PMNPs decreased 30% magnetic resonance T2 signal intensity in tumors in vivo compared to 0% decrease with PMNPs. These results indicate great potential of R11-PMPs as platform technology to target and monitor prostate cancers for diagnostic and therapeutic applications.

原文English
頁(從 - 到)3618-3625
頁數8
期刊Biomaterials
34
發行號14
DOIs
出版狀態Published - 2013 5月

All Science Journal Classification (ASJC) codes

  • 生物物理學
  • 生物工程
  • 陶瓷和複合材料
  • 生物材料
  • 材料力學

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