Protein Mimetic and Anticancer Properties of Monocyte-Targeting Peptide Amphiphile Micelles

Christopher Poon, Sampreeti Chowdhuri, Cheng-Hsiang Kuo, Yun Fang, Francis J. Alenghat, Danielle Hyatt, Kian Kani, Mitchell E. Gross, Eun Ji Chung

研究成果: Article

7 引文 (Scopus)

摘要

Monocyte chemoattractant protein-1 (MCP-1) stimulates the migration of monocytes to inflammatory sites, leading to the progression of many diseases. Recently, we described a monocyte-targeting peptide amphiphile micelle (MCP-1 PAM) incorporated with the chemokine receptor CCR2 binding motif of MCP-1, which has a high affinity for monocytes in atherosclerotic plaques. We further report here the biomimetic components of MCP-1 PAMs and the influence of the nanoparticle upon binding to monocytes. We report that MCP-1 PAMs have enhanced secondary structure compared to the MCP-1 peptide. As a result, MCP-1 PAMs displayed improved binding and chemoattractant properties to monocytes, which upregulated the inflammatory signaling pathways responsible for monocyte migration. Interestingly, when MCP-1 PAMs were incubated in the presence of prostate cancer cells in vitro, the particle displayed anticancer efficacy by reducing CCR2 expression. Given that monocytes play an important role in tumor cell migration and invasion, our results demonstrate that PAMs can improve the native biofunctional properties of the peptide and may be used as an effective inhibitor to prevent chemokine-receptor interactions that promote disease progression.

原文English
頁(從 - 到)3273-3282
頁數10
期刊ACS Biomaterials Science and Engineering
3
發行號12
DOIs
出版狀態Published - 2017 十二月 11

指紋

Amphiphiles
Chemokine CCL2
Micelles
Pulse amplitude modulation
Peptides
Proteins
Chemokine Receptors
Cells
Chemotactic Factors
Biomimetics
Tumors
Nanoparticles

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

引用此文

Poon, Christopher ; Chowdhuri, Sampreeti ; Kuo, Cheng-Hsiang ; Fang, Yun ; Alenghat, Francis J. ; Hyatt, Danielle ; Kani, Kian ; Gross, Mitchell E. ; Chung, Eun Ji. / Protein Mimetic and Anticancer Properties of Monocyte-Targeting Peptide Amphiphile Micelles. 於: ACS Biomaterials Science and Engineering. 2017 ; 卷 3, 編號 12. 頁 3273-3282.
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title = "Protein Mimetic and Anticancer Properties of Monocyte-Targeting Peptide Amphiphile Micelles",
abstract = "Monocyte chemoattractant protein-1 (MCP-1) stimulates the migration of monocytes to inflammatory sites, leading to the progression of many diseases. Recently, we described a monocyte-targeting peptide amphiphile micelle (MCP-1 PAM) incorporated with the chemokine receptor CCR2 binding motif of MCP-1, which has a high affinity for monocytes in atherosclerotic plaques. We further report here the biomimetic components of MCP-1 PAMs and the influence of the nanoparticle upon binding to monocytes. We report that MCP-1 PAMs have enhanced secondary structure compared to the MCP-1 peptide. As a result, MCP-1 PAMs displayed improved binding and chemoattractant properties to monocytes, which upregulated the inflammatory signaling pathways responsible for monocyte migration. Interestingly, when MCP-1 PAMs were incubated in the presence of prostate cancer cells in vitro, the particle displayed anticancer efficacy by reducing CCR2 expression. Given that monocytes play an important role in tumor cell migration and invasion, our results demonstrate that PAMs can improve the native biofunctional properties of the peptide and may be used as an effective inhibitor to prevent chemokine-receptor interactions that promote disease progression.",
author = "Christopher Poon and Sampreeti Chowdhuri and Cheng-Hsiang Kuo and Yun Fang and Alenghat, {Francis J.} and Danielle Hyatt and Kian Kani and Gross, {Mitchell E.} and Chung, {Eun Ji}",
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Poon, C, Chowdhuri, S, Kuo, C-H, Fang, Y, Alenghat, FJ, Hyatt, D, Kani, K, Gross, ME & Chung, EJ 2017, 'Protein Mimetic and Anticancer Properties of Monocyte-Targeting Peptide Amphiphile Micelles', ACS Biomaterials Science and Engineering, 卷 3, 編號 12, 頁 3273-3282. https://doi.org/10.1021/acsbiomaterials.7b00600

Protein Mimetic and Anticancer Properties of Monocyte-Targeting Peptide Amphiphile Micelles. / Poon, Christopher; Chowdhuri, Sampreeti; Kuo, Cheng-Hsiang; Fang, Yun; Alenghat, Francis J.; Hyatt, Danielle; Kani, Kian; Gross, Mitchell E.; Chung, Eun Ji.

於: ACS Biomaterials Science and Engineering, 卷 3, 編號 12, 11.12.2017, p. 3273-3282.

研究成果: Article

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AU - Poon, Christopher

AU - Chowdhuri, Sampreeti

AU - Kuo, Cheng-Hsiang

AU - Fang, Yun

AU - Alenghat, Francis J.

AU - Hyatt, Danielle

AU - Kani, Kian

AU - Gross, Mitchell E.

AU - Chung, Eun Ji

PY - 2017/12/11

Y1 - 2017/12/11

N2 - Monocyte chemoattractant protein-1 (MCP-1) stimulates the migration of monocytes to inflammatory sites, leading to the progression of many diseases. Recently, we described a monocyte-targeting peptide amphiphile micelle (MCP-1 PAM) incorporated with the chemokine receptor CCR2 binding motif of MCP-1, which has a high affinity for monocytes in atherosclerotic plaques. We further report here the biomimetic components of MCP-1 PAMs and the influence of the nanoparticle upon binding to monocytes. We report that MCP-1 PAMs have enhanced secondary structure compared to the MCP-1 peptide. As a result, MCP-1 PAMs displayed improved binding and chemoattractant properties to monocytes, which upregulated the inflammatory signaling pathways responsible for monocyte migration. Interestingly, when MCP-1 PAMs were incubated in the presence of prostate cancer cells in vitro, the particle displayed anticancer efficacy by reducing CCR2 expression. Given that monocytes play an important role in tumor cell migration and invasion, our results demonstrate that PAMs can improve the native biofunctional properties of the peptide and may be used as an effective inhibitor to prevent chemokine-receptor interactions that promote disease progression.

AB - Monocyte chemoattractant protein-1 (MCP-1) stimulates the migration of monocytes to inflammatory sites, leading to the progression of many diseases. Recently, we described a monocyte-targeting peptide amphiphile micelle (MCP-1 PAM) incorporated with the chemokine receptor CCR2 binding motif of MCP-1, which has a high affinity for monocytes in atherosclerotic plaques. We further report here the biomimetic components of MCP-1 PAMs and the influence of the nanoparticle upon binding to monocytes. We report that MCP-1 PAMs have enhanced secondary structure compared to the MCP-1 peptide. As a result, MCP-1 PAMs displayed improved binding and chemoattractant properties to monocytes, which upregulated the inflammatory signaling pathways responsible for monocyte migration. Interestingly, when MCP-1 PAMs were incubated in the presence of prostate cancer cells in vitro, the particle displayed anticancer efficacy by reducing CCR2 expression. Given that monocytes play an important role in tumor cell migration and invasion, our results demonstrate that PAMs can improve the native biofunctional properties of the peptide and may be used as an effective inhibitor to prevent chemokine-receptor interactions that promote disease progression.

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