Proteolysis of integrin α5 and β1 subunits involved in retinoic acid-induced apoptosis in human hepatoma Hep3B cells

Shih Lan Hsu, Chi Chih Cheng, Yi Ru Shi, Chi Wu Chiang

研究成果: Article同行評審

34 引文 斯高帕斯(Scopus)

摘要

Our previous report demonstrated that all-trans-retinoic acid (ATRA) induces detachment and death under serum starvation in several human tumor cell lines. In this study, we examined the influence of cell-extracellular matrix interaction on the ability of ATRA to induce apoptosis. Plating of human hepatoma Hep3B cells onto poly-hydroxyethylmethacrylate-coated plates in the absence of serum resulted in the acceleration of ATRA-induced apoptosis. In contrast, ATRA-induced apoptosis was significantly suppressed by plating cells onto Matrigel-coated plates but not suppressed by culturing onto collagen-, laminin-, vitronectin-, or fibronectin-coated plates. Exogenously added soluble collagen, laminin, fibronectin, vitronectin or Matrigel failed to suppress ATRA-induced apoptosis. Results from the adhesion assay indicated that the cell attachment to fibronectin was significantly inhibited by ATRA. Treatment with perturbing antibody against integrin α5 or β1 subunits resulted in promotion of ATRA-induced apoptosis. Moreover, the proteolytic cleavage of α5β1 integrin and focal adhesion kinase (FAK) proteins is linked to the early phase of the ATRA-induced apoptotic process. Furthermore, ATRA-induced detachment, death, and cleavage of α5β1 integrin and FAK were drastically suppressed by plating cells onto Matrigel-coated plates. These findings provide evidence that abrogation of cell adhesion, through proteolysis of α5β1 integrin and FAK, is closely linked to ATRA-induced apoptosis in Hep3B cells.

原文English
頁(從 - 到)193-204
頁數12
期刊Cancer Letters
167
發行號2
DOIs
出版狀態Published - 2001 6月 26

All Science Journal Classification (ASJC) codes

  • 腫瘤科
  • 癌症研究

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