Proteomic analysis of endothelial cell autoantigens recognized by anti-dengue virus nonstructural protein 1 antibodies

Hsien Jen Cheng, Chiou Feng Lin, Huan Yao Lei, Hsiao Sheng Liu, Trai Ming Yeh, Yueh Hsia Luo, Yee Shin Lin

研究成果: Article同行評審

62 引文 斯高帕斯(Scopus)

摘要

We previously showed the occurrence of autoimmune responses in dengue virus (DV) infection, which has potential implications for the pathogenesis of dengue hemorrhagic syndrome. In the present study, we have used a proteomic analysis to identify several candidate proteins on HMEC-1 endothelial cells recognized by anti-DV nonstructural protein 1 (NS1) antibodies. The target proteins, including ATP synthase β chain, protein disulfide isomerase, vimentin, and heat shock protein 60, co-localize with anti-NS1 binding sites on nonfixed HMEC-1 cells using immunohistochemical double staining and confocal microscopy. The cross-reactivity of anti-target protein antibodies with HMEC-1 cells was inhibited by NS1 protein preabsorption. Furthermore, a cross-reactive epitope on NS1 amino acid residues 311-330 (P311-330) was predicted using homologous sequence alignment. The reactivity of dengue hemorrhagic patient sera with HMEC-1 cells was blocked by synthetic peptide P311-330 pre-absorption. Taken together, our results identify putative targets on endothelial cells recognized by anti-DV NS1 antibodies, where NS1 P311-330 possesses the shared epitope.

原文English
頁(從 - 到)63-73
頁數11
期刊Experimental Biology and Medicine
234
發行號1
DOIs
出版狀態Published - 2009 1月

All Science Journal Classification (ASJC) codes

  • 一般生物化學,遺傳學和分子生物學

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