PTX3 gene activation in EGF-induced head and neck cancer cell metastasis

Wei Chiao Chang, Shuo Lun Wu, Wan Chen Huang, Jinn Yuan Hsu, Shih-Hung Chan, Ju-Ming Wang, Jhih Peng Tsai, Ben-Kuen Chen

研究成果: Article

23 引文 (Scopus)

摘要

Overexpression of the epidermal growth factor (EGF) receptor (EGFR) is associated with enhanced invasion and metastasis in head and neck squamous cell carcinoma (HNSCC). Long Pentraxin PTX3 is involved in immune escape in cancer cells. Here, we identified PTX3 as a promoting factor that mediates EGF-induced HNSCC metastasis. EGF-induced PTX3 transcriptional activation is via the binding of c-Jun to the activator protein (AP)-1 binding site of the PTX3 promoter. PI3K/Akt and NF-κB were essential for the PTX3 activation. EGF-induced PTX3 expression was blocked in c-Jun- and NF-κB-knockdown cells. EGF-mediated PTX3 secretion resulted in the enhancement of cell migration and invasion, and interactions between cancer and endothelial cells. The tail-vein injection animal model revealed that depletion of PTX3 decreased EGF-primed tumor cell metastatic seeding of the lungs. In addition, fibronectin, matrix metalloproteinase-9 (MMP9) and E-cadherin were essential components in EGFR/PTX3-mediated cancer metastasis. In conclusion, PI3K/Akt and NF-κB-dependent regulation of AP-1 mediates PTX3 transcriptional responses to EGF. Autocrine production of EGF-induced PTX3 in turn induces metastatic molecules, activating inflammatory cascades and metastasis.

原文English
頁(從 - 到)7741-7757
頁數17
期刊Oncotarget
6
發行號10
DOIs
出版狀態Published - 2015 一月 1

指紋

Head and Neck Neoplasms
Epidermal Growth Factor
Transcriptional Activation
Neoplasm Metastasis
Transcription Factor AP-1
Phosphatidylinositol 3-Kinases
Epidermal Growth Factor Receptor
Neoplasms
Matrix Metalloproteinase 9
Cadherins
Fibronectins
Protein Binding
Cell Communication
Cell Movement
Veins
Endothelial Cells
Animal Models
Binding Sites
Lung
Injections

All Science Journal Classification (ASJC) codes

  • Oncology

引用此文

Chang, Wei Chiao ; Wu, Shuo Lun ; Huang, Wan Chen ; Hsu, Jinn Yuan ; Chan, Shih-Hung ; Wang, Ju-Ming ; Tsai, Jhih Peng ; Chen, Ben-Kuen. / PTX3 gene activation in EGF-induced head and neck cancer cell metastasis. 於: Oncotarget. 2015 ; 卷 6, 編號 10. 頁 7741-7757.
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abstract = "Overexpression of the epidermal growth factor (EGF) receptor (EGFR) is associated with enhanced invasion and metastasis in head and neck squamous cell carcinoma (HNSCC). Long Pentraxin PTX3 is involved in immune escape in cancer cells. Here, we identified PTX3 as a promoting factor that mediates EGF-induced HNSCC metastasis. EGF-induced PTX3 transcriptional activation is via the binding of c-Jun to the activator protein (AP)-1 binding site of the PTX3 promoter. PI3K/Akt and NF-κB were essential for the PTX3 activation. EGF-induced PTX3 expression was blocked in c-Jun- and NF-κB-knockdown cells. EGF-mediated PTX3 secretion resulted in the enhancement of cell migration and invasion, and interactions between cancer and endothelial cells. The tail-vein injection animal model revealed that depletion of PTX3 decreased EGF-primed tumor cell metastatic seeding of the lungs. In addition, fibronectin, matrix metalloproteinase-9 (MMP9) and E-cadherin were essential components in EGFR/PTX3-mediated cancer metastasis. In conclusion, PI3K/Akt and NF-κB-dependent regulation of AP-1 mediates PTX3 transcriptional responses to EGF. Autocrine production of EGF-induced PTX3 in turn induces metastatic molecules, activating inflammatory cascades and metastasis.",
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PTX3 gene activation in EGF-induced head and neck cancer cell metastasis. / Chang, Wei Chiao; Wu, Shuo Lun; Huang, Wan Chen; Hsu, Jinn Yuan; Chan, Shih-Hung; Wang, Ju-Ming; Tsai, Jhih Peng; Chen, Ben-Kuen.

於: Oncotarget, 卷 6, 編號 10, 01.01.2015, p. 7741-7757.

研究成果: Article

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