Purification and characterization of endogenous digoxin-like immunoreactive factors in chicken blood

Jeng Shu Wei, Hung Chi Cheng, Kan Jen Tsai, Der Hui Liu, Hsuan Hsuan Lee, Daniel Tsun Yee Chiu, Tsan Zon Liu

研究成果: Article同行評審

2 引文 斯高帕斯(Scopus)

摘要

Studies have been performed to determine whether an endogenous material capable of binding to digoxin antibodies is present in the chicken plasma. In the blood of 12 chickens without feed control, endogenous digoxin-like immunoreactive factors (DLIF) binding of digoxin antibodies in enzyme immunoassays amounted to 866 ± 302 pg digoxin equivalents/mL of plasma (mean ± SEM). Immunoreactivity of DLIF increased to 1848 ± 331 pg/mL with a double value of control after boiling and acid pretreating the plasma. The major purification steps employed in this report were gel filtration column chromatography, high performance liquid chromatography (HPLC) and isoelectric focusing (IEF). Using HPLC for the separation, at least 10 chicken DLIFs with different molecular weight (MW) have been found. The MW of the smallest is 300 daltons (Da) while the largest is 100 kDa. The value of the isoelectric point of the most abundant type of DLIF from untreated chicken plasma is 6.3 as determined by IEF. The partially purified DLIF inhibits Na+,K+-ATPase from a porcine cerebral cortex as well as three human red blood cell membrane preparations in a dose-response fashion.

原文English
頁(從 - 到)1617-1629
頁數13
期刊Life Sciences
59
發行號19
DOIs
出版狀態Published - 1996 十月 4

All Science Journal Classification (ASJC) codes

  • 生物化學、遺傳與分子生物學 (全部)
  • 藥理學、毒理學和藥劑學 (全部)

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