Purification of a thermostable chitinase from Bacillus cereus by chitin affinity and its application in microbial community changes in soil

Tzu Wen Liang, Tung Yen Hsieh, San Lang Wang

研究成果: Article

15 引文 斯高帕斯(Scopus)

摘要

A thermostable chitinase was purified by chitin affinity from the culture supernatant of Bacillus cereus TKU028 with shrimp head powder (SHP) as the sole carbon/ nitrogen source. TKU028 chitinase was purified using a one-step affinity adsorbent system, and the molecular mass of TKU028 chitinase (approximately 40 kDa) was then determined using SDS-PAGE. The enzyme was stable for 60 min at temperatures below 60 °C and stable over a broad pH range of 4-9 for 60 min. In addition, the temporal changes of a bacterial community in mangrove river sediment of the Tamsui River with added SHP were also analysed by PCR-denaturing gradient gel electrophoresis to investigate the effects of B. cereus TKU028 on the degradation of SHP. The 6-week incubation sample of SHP and B. cereus TKU028-amended mangrove river sediment displayed the highest amount of biomass, reducing sugar and total sugar, and some variance of bacterial community composition existed in the soils.

原文English
頁(從 - 到)1201-1209
頁數9
期刊Bioprocess and biosystems engineering
37
發行號6
DOIs
出版狀態Published - 2014 六月

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

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