Rapid and addressable immobilization of Caenorhabditis elegans in a thermo-reversible hydrogel enabled by an optoelectric microchip

H. S. Chuang, W. Y. Chuang

研究成果: Conference contribution

摘要

Caenorhabditis elegans is an ideal model animal in genetic engineering, neural science, and developmental biology. Worm immobilization is an essential step in many operations, such as microsurgeries and fluorescent imaging, yet little has been done. To this end, an immobilization technique based on the combinative use of an optoelectric device and a thermos-reversible hydrogel, Pluronic F-127, was developed in this study. The optoelectric device was coated with a photoconductive layer, allowing local circuit channels to be rapidly switched by optical illumination. The hydrogel sandwiched in the device could reach gelation within 4 s. Notably, the gelation spot was defined by the light. The immobilized C. elegans appeared to resume its full locomotion within 3 s after the light was switched off. The technique was eventually used to assist the evaluation of senescence process of the RW1596 strain. A serial sarcomere images from the same batch of worms taken at different developmental stages showed progressive muscl e degeneration. This rapid and reversible immobilization technique provides insight to realize more worm-based applications, such as drug assays, that require long-term and constant monitoring.

原文English
主出版物標題20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
發行者Chemical and Biological Microsystems Society
頁面481-482
頁數2
ISBN(電子)9780979806490
出版狀態Published - 2016
事件20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
持續時間: 2016 十月 92016 十月 13

出版系列

名字20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
國家/地區Ireland
城市Dublin
期間16-10-0916-10-13

All Science Journal Classification (ASJC) codes

  • 控制與系統工程

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