Rapid and dynamic signal enhancement of bead-based bioassays using a multi-functional optoelectric device

K. C. Wang, F. T. Li, Dar-Bin Shieh, K. C. Kim, Han-Sheng Chuang

研究成果: Conference contribution

摘要

Colloidal particles are broadly used in biochemical applications. Considerable research works have proven particles to be useful carriers in many bead-based bioassays. The increasing use of colloidal systems raises demands for a versatile tool capable of performing multiple functions. To this end, this paper presents a simple optoelectrokinetic method for manipulating particles by simultaneously inducing electrohydrodnamic flow and electrokinetic motions. With carefully tuning the balance between several forces acting on particles, manipulation capabilities including aggregation, translation, sorting, and patterning were achieved. The experimental results showed that the technique is subjected to frequency, electric voltage, laser intensity, and particle size. For a proof of concept, the technique was used to enhance the weak signal of a dilute biotin solution by concentrating streptavidin coated particles. Compared with a ELISA reader, the minimal readout was 100 fold improvement. The demonstration provides a good insight to the future biochemical applications.

原文English
主出版物標題18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
發行者Chemical and Biological Microsystems Society
頁面1554-1556
頁數3
ISBN(電子)9780979806476
出版狀態Published - 2014 1月 1
事件18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
持續時間: 2014 10月 262014 10月 30

出版系列

名字18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
國家/地區United States
城市San Antonio
期間14-10-2614-10-30

All Science Journal Classification (ASJC) codes

  • 控制與系統工程

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