TY - GEN
T1 - Rapid and reversible immobilization of caenorhabditis elegans with an optoelectric device and pluronic F-127
AU - Chuang, Wan Yu
AU - Chuang, Han Sheng
N1 - Funding Information:
This study was supported by the Ministry of Science and Technology under the grant number 103- 2221- E-006- 172- MY2.
PY - 2016/2/26
Y1 - 2016/2/26
N2 - This paper reports a development of an optoelectric device with Pluronic (P) F-127 for reversibly immobilizing Caenorhabditis (C.) elegans. The gelation time was less than 5 s, and C. elegans also recovered from the immobilization within 10 s. The proposed device achieved addressable immobilization for long-term observation and imaging with less stress acting on worms. Assessments regarding growth, progeny, lifespan and stress were conducted. Worms imposed with simultaneous laser irradiation and an electric field in the PF-127 solution within 3 h incurred no adverse reactions. The technique is expected to benefit the research work on drug screening in the future.
AB - This paper reports a development of an optoelectric device with Pluronic (P) F-127 for reversibly immobilizing Caenorhabditis (C.) elegans. The gelation time was less than 5 s, and C. elegans also recovered from the immobilization within 10 s. The proposed device achieved addressable immobilization for long-term observation and imaging with less stress acting on worms. Assessments regarding growth, progeny, lifespan and stress were conducted. Worms imposed with simultaneous laser irradiation and an electric field in the PF-127 solution within 3 h incurred no adverse reactions. The technique is expected to benefit the research work on drug screening in the future.
UR - http://www.scopus.com/inward/record.url?scp=84970997409&partnerID=8YFLogxK
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U2 - 10.1109/MEMSYS.2016.7421742
DO - 10.1109/MEMSYS.2016.7421742
M3 - Conference contribution
AN - SCOPUS:84970997409
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 769
EP - 771
BT - MEMS 2016 - 29th IEEE International Conference on Micro Electro Mechanical Systems
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 29th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2016
Y2 - 24 January 2016 through 28 January 2016
ER -