Rapid and Sensitive Nano-Immunosensors for Botulinum

Hui Pin Cheng, Han Sheng Chuang

研究成果: Article同行評審

22 引文 斯高帕斯(Scopus)

摘要

Botulinum is a deadly bacterial toxin that causes neuroparalytic disease. However, appropriate tools to detect trace toxic proteins are scarce. This study presents a bead-based diffusometric technique for the rapid, simple, and quantitative detection of biological toxins. Functionalized particles called nano-immunosensors were fabricated by forming sandwiched immunocomplexes comprising Au nanoparticles (AuNPs), toxic proteins, and antibodies on fluorescent probe particles. Particle diffusivity tended to decline with increasing concentration of the target proteins. Calibration curves of purified botulinum toxins (0.01-500 ng/mL) were obtained from whole milk and bovine serum, and results suggested that measurement was independent of the background matrix. The activity of botulinum toxin was evaluated by coating synaptosomal-associated protein 25 (SNAP-25) on fluorescent probe particles. AuNP-conjugated antibodies attached to the probe particles when SNAP-25 proteins were cleaved by active botulinum. Thus, toxicity could be detected from slight changes in diffusivity. A short measurement time of 2 min and a limit of detection of 10 pg/mL were achieved. The nano-immunosensors demonstrated rapid biosensing capability and met the demands of onsite screening for food safety, medical instrument hygiene, and cosmetic surgery products.

原文English
頁(從 - 到)1754-1760
頁數7
期刊ACS Sensors
4
發行號7
DOIs
出版狀態Published - 2019 7月 26

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 儀器
  • 製程化學與技術
  • 流體流動和轉移過程

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