Rapid identification of bacteria and Candida pathogens in peritoneal dialysis effluent from patients with peritoneal dialysis-related peritonitis by use of multilocus PCR coupled with electrospray ionization mass spectrometry

Yu Tzu Chang, Hsuan Chen Wang, Ming Cheng Wang, An Bang Wu, Junne Ming Sung, H. Sunny Sun, Ih Jen Su, Wei Chih Kan, Chih Chiang Chien, Jyh Chang Hwang, Hsien Yi Wang, Chin Chung Tseng, Chi Jung Wu

研究成果: Article

7 引文 斯高帕斯(Scopus)

摘要

PCR coupled with electrospray ionization mass spectrometry (PCR/ESI-MS) was compared with culture for pathogen detection in peritoneal dialysis (PD)-related peritonitis. Of 21 samples of PD effluent, PCR/ESI-MS identified microorganisms in 18 (86%) samples, including Mycobacterium tuberculosis in 1 culture-negative sample. Of 15 double-positive samples, PCR/ESI-MS and culture reached levels of agreement of 100% (15/15) and 87.5% (7/8) at the genus and species levels, respectively. PCR/ESI-MS can be used for rapid pathogen detection in PD-related peritonitis.

原文English
頁(從 - 到)1217-1219
頁數3
期刊Journal of Clinical Microbiology
52
發行號4
DOIs
出版狀態Published - 2014 四月

    指紋

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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