Rapid identification of Candida albicans based on Raman spectral biosensing technology

Yong Li Pan, Tzyy Schiuan Yang, Tsung Chain Chang, Hsien Chang Chang

研究成果: Conference contribution

2 引文 斯高帕斯(Scopus)

摘要

Traditional identification methods of pathogenic Candida albicans are time-consuming due to the long-term incubation. The purpose of this study is to develop a noninvasive biomolecular sensing technology for rapid identification of Candida albicans. Surface enhanced Raman scattering (SERS) based on colloidal sliver was used to rapidly detect specific molecules on the surface of cells cultured on SDA plate. A homemade fluidic chamber was fabricated to enlarge the random sampling area, increase the path length and avoid sampling error caused by disturbance or evaporation. SERS signal was detected by a 514 nm laser with a 10x objective lens. The exposure time of CCD was 10 s. Normalization, second derivative, principal component analysis (PCA) and hierarchical cluster analysis (HCA) were also integrated for discrimination of Candida albicans by spectral patterns precisely. The results show that SERS can be used to detect high-concentration suspended cells of Candida albicans. Candida albicans can be discriminated to genus and species level by principal component analysis and hierarchical cluster analysis of high frequency features of SERS spectral patterns.

原文English
主出版物標題2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
頁面120-124
頁數5
DOIs
出版狀態Published - 2009 十二月 1
事件2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009 - Tainan, Taiwan
持續時間: 2009 十月 182009 十月 21

出版系列

名字2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009

Other

Other2009 IEEE 3rd International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2009
國家Taiwan
城市Tainan
期間09-10-1809-10-21

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

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