Rapid Identification of Yeasts Commonly Found in Positive Blood Cultures by Amplification of the Internal Transcribed Spacer Regions 1 and 2

Y. L. Li, S. N. Leaw, J. H. Chen, H. C. Chang, T. C. Chang

研究成果: Article同行評審

46 引文 斯高帕斯(Scopus)

摘要

A multiplex PCR method using one universal and eight species-specific primers was developed to rapidly identify eight yeast species found in positive blood cultures. The species-specific primers were designed from the internal transcribed spacer regions 1 and 2 of the rRNA gene, whereas the universal primer was located at the 26S rRNA gene. The eight species were Candida albicans, Candida glabrata, Candida guilliermondii, Candida krusei, Candida lusitaniae, Candida parapsilosis, Candida tropicalis, and Cryptococcus neoformans. The PCR products (116 to 630 bp) were different in length and could be effectively separated and recognized by polyacrylamide gel electrophoresis. By testing 234 positive blood cultures (237 isolates), 234 (98.7%) isolates of the above eight species were correctly identified by the multiplex PCR. The present method is simple to perform and can be completed within 6 h.

原文English
頁(從 - 到)693-696
頁數4
期刊European Journal of Clinical Microbiology and Infectious Diseases
22
發行號11
DOIs
出版狀態Published - 2003 十一月

All Science Journal Classification (ASJC) codes

  • 微生物學(醫學)
  • 傳染性疾病

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