Rapid isolation and detection of cancer cells by utilizing integrated microfluidic systems

Kang Yi Lien, Ying Hsin Chuang, Lein Yu Hung, Keng Fu Hsu, Wu Wei Lai, Chung Liang Ho, Cheng Yang Chou, Gwo Bin Lee

研究成果: Article同行評審

45 引文 斯高帕斯(Scopus)


The present study reports a new three-dimensional (3D) microfluidic platform capable of rapid isolation and detection of cancer cells from a large sample volume (e.g. ∼1 mL) by utilizing magnetic microbead-based technologies. Several modules, including a 3D microfluidic incubator for the magnetic beads to capture cancer cells, a microfluidic control module for sample transportation and a nucleic acid amplification module for genetic identification, are integrated into this microsystem. With the incorporation of surface-modified magnetic beads, target cancer cells can be specifically recognized and conjugated onto the surface of the antibody-coated magnetic microbeads by utilizing a swirling effect generated by the new 3D microfluidic incubator, followed by isolating and purifying the magnetic complexes via the incorporation of an external magnet and a microfluidic control module, which washes away any unbound waste solution. Experimental results show that over 90% of the target cancer cells can be isolated from a large volume of bio-samples within 10 min in the 3D microfluidic incubator. In addition, the expressed genes associated with ovarian and lung cancer cells can also be successfully amplified by using the on-chip nucleic acid amplification module. More importantly, the detection limit of the developed system is found to be 5 × 101 cells mL-1 for the target cancer cells, indicating that this proposed microfluidic system may be adapted for clinical use for the early detection of cancer cells. Consequently, the proposed 3D microfluidic system incorporated with immunomagnetic beads may provide a promising automated platform for the rapid isolation and detection of cancer cells with a high sensitivity.

頁(從 - 到)2875-2886
期刊Lab on a Chip
出版狀態Published - 2010 十一月 7

All Science Journal Classification (ASJC) codes

  • 生物工程
  • 生物化學
  • 化學 (全部)
  • 生物醫學工程


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