Rapid measurement of AFP using AFP-specific aptamer on a microfluidic chip

Chao Jyun Huang, Hsin I. Lin, Shu Chu Shiesh, Gwo Bin Lee

研究成果: Conference contribution

1 引文 斯高帕斯(Scopus)

摘要

This study presents a new suction-type microfluidic system capable of rapid measurement of alpha-fetoprotein (AFP) by utilizing magnetic bead-based technologies. Two modules, including a suction-type incubator for the magnetic beads to capture AFP and acridinium ester (AE)-labeled anti-AFP antibodies and a microfluidic control module for sample transportation, were integrated into this microfluidic system. With the incorporation of AFP-specific aptamer-conjugated magnetic beads, the target AFP could be recognized and attracted onto the surface of the magnetic beads from the clinical sample. The entire process including two-step incubation and purification process could be automatically performed within 25 min, which is only about 20% of the time required when using a benchtop machine (for about 130 min). Besides, the total sample and reagent volume consumed is only 105 μL, which is significantly less than that required in a large system (410 μL). More importantly, experimental results showed that clinical human serum samples can also be accurately analyzed. The microfluidic system may be promising for point-of-care applications for AFP detection in the future.

原文English
主出版物標題2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
頁面874-877
頁數4
DOIs
出版狀態Published - 2012 五月 7
事件2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
持續時間: 2012 一月 292012 二月 2

出版系列

名字Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(列印)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
國家/地區France
城市Paris
期間12-01-2912-02-02

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 凝聚態物理學
  • 機械工業
  • 電氣與電子工程

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