Rat NPFF1 receptor-mediated signaling: functional comparison of neuropeptide FF (NPFF), FMRFamide and PFR(Tic)amide

Jin Chung Chen, Wei Hsin Lee, Pei Chun Chen, Ching Ping Tseng, Eagle Yi Kung Huang

研究成果: Article同行評審

11 引文 斯高帕斯(Scopus)

摘要

Neuropeptide FF (NPFF) participates in many physiological functions associated with opioids in the mammalian CNS. We established a pheochromocytoma PC-12 cell line clone stably expressing rat NPFF1 receptors. Both NPFF and FMRFamide activated NPFF1 receptors to couple with Gi/o protein and inhibited adenylyl cyclase activity in PC-12/rNPFF1 cells, but there were no effects on MAPKs (ERK1/2 and p38 MAPK) or PI3K/Akt pathway. FMRFamide also inhibited DARPP-32/Thr34 phosphorylation in the presence of forskolin. Similarly, PFR(Tic)amide, a 'super-agonist' of NPFF receptors, inhibited the production of cAMP and slightly decreased DARPP-32/Thr34 phosphorylation in PC-12/rNPFF1 cells. Intracerebroventricular injections of PFR(Tic)amide blocked behavioral sensitization of locomotor activity to amphetamine, and intrathecal injection of PFR(Tic)amide caused a dose-dependent antinociception in vivo in rats. Thus, "over-activation" of NPFF receptors by PFR(Tic)amide induced different bio-effects from those induced by NPFF in vivo.

原文English
頁(從 - 到)1005-1014
頁數10
期刊Peptides
27
發行號5
DOIs
出版狀態Published - 2006 五月

All Science Journal Classification (ASJC) codes

  • 生物化學
  • 生理學
  • 內分泌
  • 細胞與分子神經科學

指紋

深入研究「Rat NPFF<sub>1</sub> receptor-mediated signaling: functional comparison of neuropeptide FF (NPFF), FMRFamide and PFR(Tic)amide」主題。共同形成了獨特的指紋。

引用此