Real-time photoacoustic flow cytography and photothermolysis of single circulating melanoma cells in vivo

Yun He, Lidai Wang, Junhui Shi, Junjie Yao, Lei Li, Ruiying Zhang, Chih Hsien Huang, Jun Zou, Lihong V. Wang

研究成果: Conference contribution


Metastasis is responsible for as many as 90% of cancer-related deaths, and the deadliest skin cancer, melanoma, has a high propensity for metastasis. Since hematogenous spread of circulating tumor cells (CTCs) is cancer's main route of metastasis, detecting and destroying CTCs can impede metastasis and improve patients' prognoses. Extensive studies employing exogenous agents to detect tumor-specific biomarkers and guide therapeutics to CTCs have achieved promising results, but biosafety remains a critical concern. Taking another approach, physical detection and destruction of CTCs is a safer way to evaluate and reduce metastasis risks. Melanoma cells strongly express melanosomes, providing a striking absorption contrast with the blood background in the red to near-infrared spectrum. Exploiting this intrinsic optical absorption contrast of circulating melanoma cells, we coupled dual-wavelength photoacoustic flow cytography with a nanosecond-pulsed laser killing mechanism that specifically targets melanoma CTCs. We have successfully achieved in vivo label-free imaging of rare single CTCs and CTC clusters in mice. Further, the photoacoustic signal from a CTC immediately hardware-triggers a lethal pinpoint laser irradiation that lyses it on the spot in a thermally confined manner. Our technology can facilitate early inhibition of metastasis by clearing circulating tumor cells from vasculature.

主出版物標題Photons Plus Ultrasound
主出版物子標題Imaging and Sensing 2017
編輯Alexander A. Oraevsky, Lihong V. Wang
出版狀態Published - 2017
事件Photons Plus Ultrasound: Imaging and Sensing 2017 - San Francisco, United States
持續時間: 2017 1月 292017 2月 1


名字Progress in Biomedical Optics and Imaging - Proceedings of SPIE


OtherPhotons Plus Ultrasound: Imaging and Sensing 2017
國家/地區United States
城市San Francisco

All Science Journal Classification (ASJC) codes

  • 電子、光磁材料
  • 原子與分子物理與光學
  • 放射學、核子醫學和影像學
  • 生物材料


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